Comparison of methods for DNA isolation from food samples for detection of Shiga toxin-producing Escherichia coli by real-time PCR

被引：35

作者：

Heller, LC

Davis, CR

Peak, KK

Wingfield, D

Cannons, AC

Amuso, PT

Cattani, J

机构：

[1] Tampa Gen Hosp, Dept Pathol, Miami, FL 33101 USA

[2] Univ S Florida, Coll Publ Hlth, Ctr Biol Def, Tampa, FL 33612 USA

[3] Florida Dept Hlth, Bur Labs Tampa, Tampa, FL 33612 USA

来源：

APPLIED AND ENVIRONMENTAL MICROBIOLOGY | 2003年 / 69卷 / 03期

关键词：

D O I：

10.1128/AEM.69.3.1844-1846.2003

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

In this study, food samples were intentionally contaminated with Escherichia coli O157:H7, and then DNA was isolated by using four commercial kits. The isolated DNA samples were compared by using real-time PCR detection of the Shiga toxin genes. The four kits tested worked similarly.

引用

页码：1844 / 1846

页数：3

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