Synergistic activation of seed storage protein gene expression in Arabidopsis by ABI3 and two bZIPs related to OPAQUE2

被引:148
作者
Lara, P [1 ]
Oñate-Sánchez, L [1 ]
Abraham, Z [1 ]
Ferrándiz, C [1 ]
Díaz, I [1 ]
Carbonero, P [1 ]
Vicente-Carbajosa, J [1 ]
机构
[1] ETS Ingn Agron, Lab Bioquim & Biol Mol, Dept Biotecnol UPM, Madrid 28040, Spain
关键词
D O I
10.1074/jbc.M210538200
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The expression of many seed storage protein genes in cereals relies on transcription factors of the bZIP class, belonging to the maize OPAQUE2 family. Here, we describe a survey of such factors in the genome of Arabidopsis thaliana, and the characterization of two of them, AtbZIP10 and AtbZIP25. Expression analysis by in situ hybridization shows that the occurrence of their mRNAs in the seed starts from early stages of development, peaks at maturation, and declines later in seed development, matching temporally and spatially those of the seed storage protein genes encoding 2S albumins and cruciferins. Gel mobility shift assays showed that AtbZIP10 and AtbZIP25 bind the ACGT boxes present in At2S and CRU3 promoters. Moreover, using the yeast two-hybrid system we show that AtbZIP10 and AtbZIP25 can interact in vivo with ABI3, an important regulator of gene expression in the seed of Arabidopsis. Transient expression analyses of a reporter gene under the control of the At2S1 promoter in transgenic plants overexpressing ectopically AtbZIP10, AtbZIP25, and ABI3 reveal that none of these factors could activate significantly the reporter gene when expressed individually. However, co-expression of AtbZIP10/25 with ABI3 resulted in a remarkable increase in the activation capacity over the At2S1 promoter, suggesting that they are part of a regulatory complex involved in seed-specific expression. This study shows a common mechanism of ABI3 in regulating different seed-specific genes through combinatorial interactions with particular bZIP proteins and a conserved role of O2-like bZIPs in monocot and dicot species.
引用
收藏
页码:21003 / 21011
页数:9
相关论文
共 52 条
[1]   The wheat transcriptional activator SPA: A seed-specific bZIP protein that recognizes the GCN4-like motif in the bifactorial endosperm box of prolamin genes [J].
Albani, D ;
HammondKosack, MCU ;
Smith, C ;
Conlan, S ;
Colot, V ;
Holdsworth, M ;
Bevan, MW .
PLANT CELL, 1997, 9 (02) :171-184
[2]   Analysis of the genome sequence of the flowering plant Arabidopsis thaliana [J].
Kaul, S ;
Koo, HL ;
Jenkins, J ;
Rizzo, M ;
Rooney, T ;
Tallon, LJ ;
Feldblyum, T ;
Nierman, W ;
Benito, MI ;
Lin, XY ;
Town, CD ;
Venter, JC ;
Fraser, CM ;
Tabata, S ;
Nakamura, Y ;
Kaneko, T ;
Sato, S ;
Asamizu, E ;
Kato, T ;
Kotani, H ;
Sasamoto, S ;
Ecker, JR ;
Theologis, A ;
Federspiel, NA ;
Palm, CJ ;
Osborne, BI ;
Shinn, P ;
Conway, AB ;
Vysotskaia, VS ;
Dewar, K ;
Conn, L ;
Lenz, CA ;
Kim, CJ ;
Hansen, NF ;
Liu, SX ;
Buehler, E ;
Altafi, H ;
Sakano, H ;
Dunn, P ;
Lam, B ;
Pham, PK ;
Chao, Q ;
Nguyen, M ;
Yu, GX ;
Chen, HM ;
Southwick, A ;
Lee, JM ;
Miranda, M ;
Toriumi, MJ ;
Davis, RW .
NATURE, 2000, 408 (6814) :796-815
[3]   The homologous ABI5 and EEL transcription factors function antagonistically to fine-tune gene expression during late embryogenesis [J].
Bensmihen, S ;
Rippa, S ;
Lambert, G ;
Jublot, D ;
Pautot, V ;
Granier, F ;
Giraudat, J ;
Parcy, F .
PLANT CELL, 2002, 14 (06) :1391-1403
[4]   bZIP and DOF transcription factors in the regulation of gene expression in barley endosperm [J].
Carbonero, P ;
Vicente-Carbajosa, JV ;
Mena, M ;
Oñate, L ;
Lara, P ;
Diaz, I .
SEED BIOLOGY: ADVANCES AND APPLICATIONS, 2000, :27-41
[5]   Oct-1 POU and octamer DNA co-operate to recognise the Bob-1 transcription co-activator via induced folding [J].
Chang, JF ;
Phillips, K ;
Lundbäck, T ;
Gstaiger, M ;
Ladbury, JE ;
Luisi, B .
JOURNAL OF MOLECULAR BIOLOGY, 1999, 288 (05) :941-952
[6]   ABFs, a family of ABA-responsive element binding factors [J].
Choi, HI ;
Hong, JH ;
Ha, JO ;
Kang, JY ;
Kim, SY .
JOURNAL OF BIOLOGICAL CHEMISTRY, 2000, 275 (03) :1723-1730
[7]   The 22 bp W1 element in the pea lectin promoter is necessary and, as a multimer, sufficient for high gene expression in tobacco seeds [J].
dePater, S ;
Pham, K ;
Klitsie, I ;
Kijne, J .
PLANT MOLECULAR BIOLOGY, 1996, 32 (03) :515-523
[8]   The GAMYB protein from barley interacts with the DOF transcription factor BPBF and activates endosperm-specific genes during seed development [J].
Diaz, I ;
Vicente-Carbajosa, J ;
Abraham, Z ;
Martínez, M ;
Isabel-La Moneda, I ;
Carbonero, P .
PLANT JOURNAL, 2002, 29 (04) :453-464
[9]   Rapid stimulation of a soybean protein-serine kinase that phosphorylates a novel bZIP DNA-binding protein, G/HBF-1, during the induction of early transcription-dependent defenses [J].
DrogeLaser, W ;
Kaiser, A ;
Lindsay, W ;
Halkier, BA ;
Loake, GJ ;
Doerner, P ;
Dixon, RA ;
Lamb, C .
EMBO JOURNAL, 1997, 16 (04) :726-738
[10]   Functional dissection of a napin gene promoter: Identification of promoter elements required for embryo and endosperm-specific transcription [J].
Ellerstrom, M ;
Stalberg, K ;
Ezcurra, I ;
Rask, L .
PLANT MOLECULAR BIOLOGY, 1996, 32 (06) :1019-1027