Intracellular integration of synthetic nanostructures with viable cells for controlled biochemical manipulation

被引:145
作者
McKnight, TE [1 ]
Melechko, AV
Griffin, GD
Guillorn, MA
Merkulov, VI
Serna, F
Hensley, DK
Doktycz, MJ
Lowndes, DH
Simpson, ML
机构
[1] Oak Ridge Natl Lab, Mol Scale Engn & Nanoscale Technol Res Grp, Oak Ridge, TN 37831 USA
[2] Univ Tennessee, Dept Elect & Comp Engn, Knoxville, TN 37996 USA
[3] Oak Ridge Natl Lab, Div Life Sci, Oak Ridge, TN 37831 USA
[4] Oak Ridge Natl Lab, Condensed Matter Sci Div, Oak Ridge, TN 37831 USA
[5] Univ Tennessee, Dept Mat Sci & Engn, Knoxville, TN 37996 USA
关键词
D O I
10.1088/0957-4484/14/5/313
中图分类号
TB3 [工程材料学];
学科分类号
0805 ; 080502 ;
摘要
We demonstrate the integration of vertically aligned carbon nanofibre (VACNF) elements with the intracellular domains of viable cells for controlled biochemical manipulation. Deterministically synthesized VACNFs were modified with either adsorbed or covalently-linked plasmid DNA and were subsequently inserted into cells. Post insertion viability of the cells was demonstrated by continued proliferation of the interfaced cells and long-term (>22 day) expression of the introduced plasmid. Adsorbed plasmids were typically desorbed in the intracellular domain and segregated to progeny cells. Covalently bound plasmids remained tethered to nanofibres and were expressed in interfaced cells but were not partitioned into progeny, and gene expression ceased when the nanofibre was no longer retained. This provides a method for achieving a genetic modification that is non-inheritable and whose extent in time can be directly and precisely controlled. These results demonstrate the potential of VACNF arrays as an intracellular interface for monitoring and controlling subcellular and molecular phenomena within viable cells for applications including biosensors, in vivo diagnostics, and in vivo logic devices.
引用
收藏
页码:551 / 556
页数:6
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