Ultra-sensitive fluorescence reader for bioanalysis

被引：9

作者：

Hesse, J

Sonnleitner, M

Schütz, GJ

机构：

[1] Johannes Kepler Univ Linz, Inst Biophys, A-4040 Linz, Austria

[2] Upper Austrian Res GmbH, Ctr Biomed Nanotechnol, A-4020 Linz, Austria

来源：

CURRENT PHARMACEUTICAL BIOTECHNOLOGY | 2004年 / 5卷 / 03期

关键词：

single molecule microscopy; scanning device; biochip reader; lipid bilayer; FRET; lateral diffusion; in vivo; electrophysiology;

D O I：

10.2174/1389201043376850

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Recent advances in the development of new microscopical techniques with single-molecule sensitivity have given access to essentially new types of information on biological systems. In this review, basic methodological Concepts Of Ultra-sensitive microscopy are presented and characterized, with focus on their applicability for a bioanalytical instrument. Measurements on artificial lipid bilayers were used to evaluate the feasibility of this novel technology. First examples of single molecule microscopy on cell membranes revealed new basic insights into the lateral organization of the plasma membrane.

引用

页码：309 / 319

页数：11

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