IMMUNOMODULATORY ACTIVITY OF A PLANT EXTRACT CONTAINING HUMAN PAPILLOMAVIRUS 16-E7 PROTEIN IN HUMAN MONOCYTE-DERIVED DENDRITIC CELLS

被引：25

作者：

Di Bonito, P. ^{[1
]}

Grasso, F. ^{[1
]}

Mangino, G. ^{[2
]}

Massa, S. ^{[3
]}

Illiano, E. ^{[3
]}

Franconi, R. ^{[3
]}

Fanales-Belasio, E. ^{[4
]}

Falchi, M. ^{[4
]}

Affabris, E. ^{[2
]}

Giorgi, C. ^{[1
]}

机构：

[1] Ist Super Sanita, Dept Infect Parasit & Immune Mediated Dis, I-00161 Rome, Italy

[2] Univ Roma Tre, Dept Biol, Rome, Italy

[3] BAS BIOTEC GEN, ENEA, Italian Natl Agcy New Technol Energy & Environm, Rome, Italy

[4] Ist Super Sanita, Natl Ctr AIDS, I-00161 Rome, Italy

来源：

INTERNATIONAL JOURNAL OF IMMUNOPATHOLOGY AND PHARMACOLOGY | 2009年 / 22卷 / 04期

关键词：

HPV16; E7; protein; dendritic cells; plant extract; plant vaccine; PENETRATING PEPTIDES; IN-VITRO; ANTIGEN; MATURATION; LYMPHOCYTES; VACCINATION; STRATEGIES; INDUCTION; VACCINES; CANCER;

D O I：

10.1177/039463200902200412

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

This study reports the immunomodulatory activity on human Monocyte Derived Dendritic Cells (MDDCs) of a vaccine preparation shown to be effective against an HPV16-related tumour in an animal model. The vaccine is composed of extract from Nicotiana benthamiana leaves containing HPV16 E7 protein expressed by a potato virus X-derived vector (NbPVX-E7). The effect of the extract was evaluated on MDDC differentiation and maturation by monitoring the phenotypic expression of specific markers. The results show that NbPVX-E7 does not induce monocyte differentiation to dendritic cells, but does induce MDDC maturation. Plant extract does not influence MDDC-uptake of E7-FITC while it significantly improves the Ovalbumin-FITC uptake, considered as a model antigen. Importantly, NbPVX-E7-pulsed MDDCs/PBMCs are able to prime human blood-derived lymphocytes from healthy individuals to induce HPV16 E7-specific cytotoxic activity. This is a propaedeutic study for a possible use of E7-containing plant extract in human immunotherapy of HPV-related lesions.

引用

页码：967 / 978

页数：12

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