Rapid purification of His6-tagged Bacillus subtilis core RNA polymerase

被引：30

作者：

Anthony, LC

Artsimovitch, I

Svetlov, V

Landick, R

Burgess, RR ^{[1
]}

机构：

[1] Univ Wisconsin, Mcardle Lab Canc Res, Madison, WI 53706 USA

[2] Univ Wisconsin, Dept Bacteriol, Madison, WI 53706 USA

来源：

PROTEIN EXPRESSION AND PURIFICATION | 2000年 / 19卷 / 03期

关键词：

D O I：

10.1006/prep.2000.1272

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Bacillus subtilis core RNA polymerase, containing a His(6)-fusion to the C-terminus of the beta' subunit, was isolated by Ni-NTA, Superdex 200 gel filtration, and Mono Q anion-exchange chromatography. The purified core enzyme was shown to be free of the major sigma factor sigma(A) and the transcription factors NusA and GreA. The purification procedure can be completed within 1 working day, is scalable, and yields highly purified and active core RNA polymerase. (C) 2000 Academic Press.

引用

页码：350 / 354

页数：5

共 15 条

[1] Pausing by bacterial RNA polymerase is mediated by mechanistically distinct classes of signals [J].