Nerve growth factor, β3-adrenoceptor and uncoupling protein 1 expression in rat brown fat during postnatal development

被引：23

作者：

Nisoli, E ^{[1
]}

Tonello, C ^{[1
]}

Carruba, MO ^{[1
]}

机构：

[1] Univ Milan, Sch Med, Osped L Sacco,Dept Pharmacol Chemotherapy & Med T, Ctr Study & Res Obes,LITA Vialba, I-20157 Milan, Italy

来源：

NEUROSCIENCE LETTERS | 1998年 / 246卷 / 01期

关键词：

brown adipose tissue; norepinephrine; uncoupling protein 1; beta(3)-adrenoceptor; non-shivering thermogenesis; development; trophic factor;

D O I：

10.1016/S0304-3940(98)00220-1

中图分类号：

Q189 [神经科学];

学科分类号：

071006 [神经生物学];

摘要：

An analysis was made of the expression of nerve growth factor (NGF) mRNA and protein in the brown fat of rats at different ages, and the results compared with the expression of beta(3)-adrenoceptor and uncoupling protein 1 (UCP1). NGF, beta(3)-adrenoceptor, and UCP1 messenger RNA and protein levels were measured by means of reverse transcription-polymerase chain reaction (RT-PCR) and Western blotting in the brown fat of rats at different ages (from 20-day-old fetuses (E20) to 16-month-old rats). During the perinatal period, NGF production increased and then declined to adult levels (which are comparable with fetal levels) by eight months, and remained stable thereafter. Relatively low levels of NGF were present in the brown fat of aged rats. Taken together, these results suggest that NGF may be responsible for regulating sympathetic innervation during the perinatal and adult periods. (C) 1998 Elsevier Science Ireland Ltd.

引用

页码：5 / 8

页数：4

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