Acute differential regulation by corticosteroids of epithelial sodium channel subunit and Nedd4 mRNA levels in the distal colon

被引:26
作者
Fuller, PJ [1 ]
Brennan, FE [1 ]
Burgess, JS [1 ]
机构
[1] Prince Henrys Inst Med Res, Clayton, Vic 3168, Australia
来源
PFLUGERS ARCHIV-EUROPEAN JOURNAL OF PHYSIOLOGY | 2000年 / 441卷 / 01期
关键词
aldosterone; dexamethasone; ENaC; glucocorticoid; mineralocorticoid;
D O I
10.1007/s004240000366
中图分类号
Q4 [生理学];
学科分类号
071003 ;
摘要
The molecular mechanisms by which corticosteroids affect fluid and electrolyte balance have yet to be fully elucidated. The apical amiloride-sensitive electrogenic epithelial sodium channel (ENaC) has been shown to have a central role in corticosteroid-mediated sodium transport in the distal colon. The acute response of the alpha-, beta- and gamma ENaC subunit genes to a single parenteral dose of aldosterone or dexamethasone was examined in the rat distal colon in vivo. The response of the Nedd4 gene, whose product is involved in channel turnover, was also examined. Whilst the alpha ENaC and Nedd4 genes showed no significant response to either steroid, both the beta- and gamma ENaC mRNA levels were increased acutely by both aldosterone and dexamethasone. The gamma ENaC mRNA appears to have a very short half-life. Use of the highly selective glucocorticoid receptor agonist RU28362 confirmed that the response was mediated by both the mineralocorticoid and glucocorticoid receptors.
引用
收藏
页码:94 / 101
页数:8
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