Alternatively spliced human tissue factor (asHTF) is not pro-coagulant

被引：58

作者：

Censarek, Petra

Bobbe, Anett

Grandoch, Maria

Schroer, Karsten

Weber, Artur-Aron

机构：

[1] Univ Duisburg Gesamthsch, Inst Pharmakol, Univ Klinikum Essen, D-45122 Essen, Germany

[2] Univ Klinikum Dusseldorf, Inst Pharmakol & Klin Pharmakol, D-45122 Dusseldorf, Germany

来源：

THROMBOSIS AND HAEMOSTASIS | 2007年 / 97卷 / 01期

关键词：

coagulation factors; tissue factor / factorVII; thrombosis;

D O I：

10.1160/TH06-09-0524

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

It has been proposed that alternatively-spliced human tissue factor (asHTF) is pro-coagulant. We have evaluated the function of asHTF in a mammalian expression system. Full-length human tissue factor (HTF) and asHTF were cloned from smooth muscle cells and over-expressed in HEK293 cells. As expected, a marked pro-coagulant activity (FX activation, thrombin generation) was observed on the surface, in lysates, and on microparticles from HTF transfected cells. In contrast, no pro-coagulant activity of asHTF was observed.

引用

页码：11 / 14

页数：4

共 20 条

[1] Identification and characterization of murine alternatively spliced tissue factor [J].