Poly(A) cDNA-specific (PACS) RT-PCR: A quantitative method for the measurement of any poly(A)-containing mRNA not affected by contaminating genomic DNA

被引：9

作者：

Folz, RJ ^{[1
]}

Nepluev, I ^{[1
]}

机构：

[1] Duke Univ, Med Ctr, Durham, NC 27710 USA

来源：

BIOTECHNIQUES | 2000年 / 29卷 / 04期

关键词：

D O I：

10.2144/00294st01

中图分类号：

Q5 [生物化学];

学科分类号：

071010 [生物化学与分子生物学]; 081704 [应用化学];

摘要：

We present a simple and efficient RT-PCR method for the detection and quantitation of any poly(A)-containing mRNA that is not affected by contaminating genomic DNA and does not rely on exhaustive DNase digestion protocols. The technique described here requires the use of an antisense primer designed to contain 6-8 bp cDNA-specific sequence and an additional 17 Ts located on the 5' end to take advantage of the poly(A) tail. A second cDNA-specific sense primer can be used that does not need to be separated by intronic DNA sequence.

引用

页码：762 / +

页数：5

共 5 条

[1]

Mouse extracellular superoxide dismutase: Primary structure, tissue-specific gene expression, chromosomal localization, and lung in situ hybridization [J].