Purification and characterization of two hemolysins from Stichodactyla helianthus

被引：110

作者：

Lanio, ME

Morera, V

Alvarez, C

Tejuca, M

Gómez, T

Pazos, F

Besada, V

Martínez, D

Huerta, V

Padrón, G

Chávez, MD

机构：

[1] Ctr Genet Engn & Biotechnol, Prot Struct Dept, Phys Chem Div, Havana, Cuba

[2] Univ Havana, Fac Biol, Dept Biochem, Havana, Cuba

来源：

TOXICON | 2001年 / 39卷 / 2-3期

关键词：

hemolysin; cytolysin; sea anemone; Stichodactyla helianthus;

D O I：

10.1016/S0041-0101(00)00106-9

中图分类号：

R9 [药学];

学科分类号：

1007 ;

摘要：

Two hemolysins, Sticholysin I (St I) and Sticholysin II (St II) were purified from the sea anemone Stichodactyla helianthus combining gel filtration and ion exchange chromatography. The amino acid composition of both cytolysins was determined revealing a high proportion of glycine, lysine, tyrosine and non-polar amino acids (alanine, leucine and valine). Cysteine was not found in either polypeptide. Molecular masses of St I and St II were 19401 and 19290 Da, respectively. N-terminal sequence analysis of St I and St II showed a high homology between them suggesting they are isoforms of the same cytolysin. Compared with other sea anemone cytolysins, St I and St II contain a 22 amino acid insertion fragment also present in Eq T II/Tn C and probably in CaT I and Hm T and absent in C III, the major hemolysin previously reported in this anemone. (C) 2000 Elsevier Science Ltd. All rights reserved.

引用

页码：187 / 194

页数：8

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