Effect of combined treatment with perindoprilat and low-power red light laser irradiation on human erythrocyte membrane fluidity, membrane potential and acetylcholinesterase activity

被引：16

作者：

Piasecka, A ^{[1
]}

Leyko, W ^{[1
]}

Krajewska, E ^{[1
]}

Bryszewska, M ^{[1
]}

机构：

[1] Univ Lodz, Dept Gen Biophys, PL-90237 Lodz, Poland

来源：

SCANDINAVIAN JOURNAL OF CLINICAL & LABORATORY INVESTIGATION | 2000年 / 60卷 / 05期

关键词：

effect of combined action of perindoprilat and laser irradiation; membrane fluidity; membrane potential; acetcholinesterase activity;

D O I：

10.1080/003655100750019305

中图分类号：

R-3 [医学研究方法]; R3 [基础医学];

学科分类号：

1001 ;

摘要：

Erythrocyte membrane fluidity, membrane potential and acetylcholinesterase activity were estimated after in vitro combined treatment of human erythrocytes with perindoprilat and low-power red light irradiation. Membrane fluidity was determined using fluorescent labels spectroscopy; membrane potential was evaluated by means of potential-sensitive fluorescent dyes; and acetylcholinesterase activity was estimated using the Ellman method. Both perindoprilat and laser irradiation, when used separately, increase microviscosity in the polar region and hyperpolarize the membranes in comparison with control erythrocytes. The combined action of these agents does not cause any further change in these parameters. Perindoprilat has an additional inhibitory effect on the activity of acetylcholinesterase, whereas laser irradiation causes an increase in the activity of the enzyme. Their combined action restores the initial activity of the enzyme independently of the sequence of treatment with both agents.

引用

页码：395 / 402

页数：8

共 24 条

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