Amplified fragment length polymorphism analysis of Campylobacter jejuni strains isolated from chickens and from patients with gastroenteritis or Guillain-Barre or Miller Fisher syndrome

被引:47
作者
Duim, B
Ang, CW
van Belkum, A
Rigter, A
van Leeuwen, NWJ
Endtz, HP
Wagenaar, JA
机构
[1] Inst Anim Sci & Hlth, Dept Bacteriol, NL-8200 AB Lelystad, Netherlands
[2] Erasmus Univ Ctr, Dept Neurol & Immunol, Rotterdam, Netherlands
[3] Erasmus Univ Ctr, Dept Med Microbiol & Infect Dis, Rotterdam, Netherlands
[4] Natl Inst Publ Hlth & Environm, NL-3720 BA Bilthoven, Netherlands
关键词
D O I
10.1128/AEM.66.9.3917-3923.2000
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
The high-resolution genotyping method of amplified fragment length polymorphism (AFLP) analysis was used to study the genetic relationships between Campylobacter jejuni strains infecting chickens (n = 54) and those causing gastroenteritis in humans (n = 53). In addition, C. jejuni strains associated with the development of Guillain-Barre syndrome (GBS) (n = 14) and Miller Fisher syndrome (MFS) (n = 4), two related acute paralytic syndromes in human, were included. Strains were isolated between 1989 and 1998 in The Netherlands. The AFLP banding patterns were analyzed with correlation-based and band-based similarity coefficients and UPGMA (unweighted pair group method using average linkages) cluster analysis. All C, jejuni strains shelved highly heterogeneous fingerprints, and no fingerprints exclusive for chicken strains or for human strains were obtained. All strains were separated in two distinct genetic groups. In group A the percentage of human strains was significantly higher and may be an indication that genotypes of this group are more frequently associated with human diseases, We conclude that C. jejuni from chickens cannot be distinguished from human strains and that GBS or MFS related strains do not belong to a distinct genetic group.
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收藏
页码:3917 / 3923
页数:7
相关论文
共 28 条
[1]   POPULATION-GENETICS OF HUMAN AND ANIMAL ENTERIC CAMPYLOBACTER STRAINS [J].
AESCHBACHER, M ;
PIFFARETTI, JC .
INFECTION AND IMMUNITY, 1989, 57 (05) :1432-1437
[2]  
ANG CW, 1998, J NEUROL, V245, P417
[3]   Computer-assisted analysis and epidemiological value of genotyping methods for Campylobacter jejuni and Campylobacter coli [J].
de Boer, P ;
Duim, B ;
Rigter, A ;
van der Plas, J ;
Jacobs-Reitsma, WF ;
Wagenaar, JA .
JOURNAL OF CLINICAL MICROBIOLOGY, 2000, 38 (05) :1940-1946
[4]  
DEWIT MAS, 1999, 216852003 RIVM NIVEL
[5]  
Duim B, 1999, APPL ENVIRON MICROB, V65, P2369
[6]  
Endtz HP, 2000, J CLIN MICROBIOL, V38, P2297
[7]   A 2-YEAR STUDY OF THE DISTRIBUTION OF THERMOPHILIC CAMPYLOBACTERS IN HUMAN, ENVIRONMENTAL AND FOOD SAMPLES FROM THE READING AREA WITH PARTICULAR REFERENCE TO TOXIN PRODUCTION AND HEAT-STABLE SEROTYPE [J].
FRICKER, CR ;
PARK, RWA .
JOURNAL OF APPLIED BACTERIOLOGY, 1989, 66 (06) :477-490
[8]   Restriction fragment length polymorphism analysis and random amplified polymorphic DNA analysis of Campylobacter jejuni strains isolated from patients with Guillain-Barre syndrome [J].
Fujimoto, S ;
Allos, BM ;
Misawa, N ;
Patton, CM ;
Blaser, MJ .
JOURNAL OF INFECTIOUS DISEASES, 1997, 176 (04) :1105-1108
[9]  
HEUVELINK AE, 1999, 285859 RIVM
[10]   Cross-reactive antibodies against gangliosides and Campylobacter jejuni lipopolysaccharides in patients with Guillain-Barre or Miller Fisher syndrome [J].
Jacobs, BC ;
Hazenberg, MP ;
vanDoorn, PA ;
Endtz, HP ;
vanderMeche, FGA .
JOURNAL OF INFECTIOUS DISEASES, 1997, 175 (03) :729-733