In vitro selection of structure-switching signaling aptamers

被引：342

作者：

Nutiu, R

Li, YF ^{[1
]}

机构：

[1] McMaster Univ, Dept Biochem, Hamilton, ON L8N 3Z5, Canada

[2] McMaster Univ, Dept Chem, Hamilton, ON L8N 3Z5, Canada

来源：

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION | 2005年 / 44卷 / 07期

关键词：

aptamers; fluorescence; in vitro selection; nucleic acids; sensors;

D O I：

10.1002/anie.200461848

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Picking out probes: A novel approach permits isolation of standard DNA aptamers by in vitro selection and conversion into fluorescence signaling probes. This method comprises the isolation of DNA aptamers capable of duplex-to-complex structure switching and labeling the derived aptamers with a pair of short DNA strands with a fluorophore F and a quencher Q to create a reporter system for real-time sensing (see picture). © 2005 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim.

引用

页码：1061 / 1065

页数：5

共 26 条

[1] Aptamers switch on fluorescence of triphenylmethane dyes [J].