Modulation by soluble factors of gelatinase activities released by osteoblastic cells

This study investigated the ability of normal human osteoblasts (hOb) and osteogenic sarcoma cells (MG-63 and SaOS2) to produce gelatinases and undergo modulation by interleukin 1 beta (IL-1 beta), interleukin 6 (IL-6), oncostatin M (OSM), leukaemia inhibitory factor (LIF), growth hormone (GH) and insulin-like growth factor-I (IGF-I), Gelatinase activities were determined by zymogaphy, and a quantitative analysis was performed by ELISA, The MMP-2 activities of the three cell lines were significantly increased in the presence of IL-1 beta and IL-6, but no modulation of MMP-2 activities was observed in the presence of OSM, LIF and GH, IGF-I increased the activity released by SaOS2 and hOb, but no modulation was detectable in MG-63 cell conditioned medium, An upmodulation of pro-MMP-2 secretion by SaOS2 and hOb was observed for all soluble factors used, whereas an upmodulation of pro-MMP-2 secretion by MG-63 was observed only in the presence of IL-1 beta, IL-6 and IGF-I, Thus, osteoblastic cells modulated by cytokines can be involved in bone resorption as a result of the protease activities released. (C) 2000 Academic Press.