Transport of a genetically modified Pseudomonas fluorescens and its parent strain through undisturbed tropical soil cores

被引:8
作者
Guimares, VF
Cruz, IV
Hagler, AN
Mendonca-Hagler, LC
van Elsas, JD
机构
[1] Univ Fed Rio de Janeiro, CCS, Inst Microbiol Prof Paulo Goes, BR-21941590 Ilha Fundao, RJ, Brazil
[2] DLO, IPO, NL-6700 GW Wageningen, Netherlands
关键词
GMO; Pseudomonas fluorescens; transport; tropical soil;
D O I
10.1016/S0929-1393(97)00024-3
中图分类号
S15 [土壤学];
学科分类号
0903 ; 090301 ;
摘要
The transport of a genetically modified strain of Pseudonronas fluorescens, BR12, and its parent, BR5, was studied after irrigation of undisturbed clayey and sandy soil cores, simulating heavy tropical rainfall (56.6 mm/h). Vertical transport of both inoculant strains was detected in all soil cores. Transport was negatively affected by the organic matter content of soil, whereas soil texture did not affect the degree of transport. Both factors influenced the survival of the introduced bacteria, in particular that of the modified strain. The initial moisture and structural status of the clayey soil, which was related to seasonal influences, also influenced the degree of bacterial translocation. In cores with two clay soil types, the genetically modified strain was transported to a greater extent than its parent. However, the modified strain showed similar or lower survival rates than its parent in topsoil layers of the different clay soil type and the sandy soil cores. Counts of culturable cells of both inoculant strains in the percolation water remained roughly stable or decreased during the experiment, and the inoculant survival was clearly affected by the soil from which the percolation water was obtained. Both strains showed good survival in the percolation water from microcosms-containing clay soil enriched with manure to about 6% organic matter, whereas their population sizes declined in percolation water from non-manured clay or sandy soil cores. The results suggested that the release of derivatives of P. fluorescens strain BR5 into soils in tropical climates should be carefully planned and monitored in order to guarantee their efficacy as biocontrol agents and to assess the potential contamination of ground and surface waters. (C) 1997 Elsevier Science B.V.
引用
收藏
页码:41 / 50
页数:10
相关论文
共 32 条
[1]  
ARAUJO MAV, 1994, FEMS MICROBIOL ECOL, V13, P205, DOI 10.1111/j.1574-6941.1994.tb00067.x
[2]  
ARAUJO MAV, 1995, REV MICROBIOL, V26, P6
[3]  
ARAUJO MAV, 1996, WORLD J MICROB BIOT, V12, P1
[4]   PLASMID EXPRESSION AND MAINTENANCE DURING LONG-TERM STARVATION-SURVIVAL OF BACTERIA IN WELL WATER [J].
CALDWELL, BA ;
YE, C ;
GRIFFITHS, RP ;
MOYER, CL ;
MORITA, RY .
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1989, 55 (08) :1860-1864
[5]   VIABLE BUT NON-CULTURABLE VIBRIO-CHOLERAE AND RELATED PATHOGENS IN THE ENVIRONMENT - IMPLICATIONS FOR RELEASE OF GENETICALLY ENGINEERED MICROORGANISMS [J].
COLWELL, RR ;
BRAYTON, PR ;
GRIMES, DJ ;
ROSZAK, DB ;
HUQ, SA ;
PALMER, LM .
BIO-TECHNOLOGY, 1985, 3 (09) :817-820
[6]   SURVIVAL OF RIFAMPIN-RESISTANT MUTANTS OF PSEUDOMONAS-FLUORESCENS AND PSEUDOMONAS-PUTIDA IN SOIL SYSTEMS [J].
COMPEAU, G ;
ALACHI, BJ ;
PLATSOUKA, E ;
LEVY, SB .
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1988, 54 (10) :2432-2438
[7]  
CROZAT Y, 1987, BIOL FERT SOILS, V4, P85
[8]   QUANTITATIVE AND QUALITATIVE STUDIES OF PHYLLOPLANE BACTERIA FROM LOLIUM-PERENNE [J].
DICKINSON, CH ;
AUSTIN, B ;
GOODFELLOW, M .
JOURNAL OF GENERAL MICROBIOLOGY, 1975, 91 (NOV) :157-166
[9]  
Elsas J. D. van, 1991, Biological monitoring of genetically engineered plants and microbes. Proceedings of the Kiawah Island Conference, South Carolina, USA, 27-30 November 1990., P67
[10]  
GERBA CP, 1985, GROUND WATER QUALITY, P53