Reduced MK801 binding in neocortical neurons after AAV-mediated transfections with NMDA-R1 antisense cDNA

被引：5

作者：

Shafron, DR

Simpkins, CE

Jebelli, B

Day, AL

Meyer, EM

机构：

[1] Univ Florida, J Hillis Miller Hlth Ctr, Coll Med, Dept Pharmacol, Gainesville, FL 32610 USA

[2] Univ Florida, J Hillis Miller Hlth Ctr, Coll Med, Dept Neurosurg, Gainesville, FL 32610 USA

来源：

BRAIN RESEARCH | 1998年 / 784卷 / 1-2期

关键词：

pTRUF3-aR1; pJDT95dlk-aR1; adeno-associated virus;

D O I：

10.1016/S0006-8993(97)01029-9

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

Two adeno-associated virus (AAV)-derived plasmids were constructed with portions of the N-methyl-D-aspartic acid-R1 (NMDA-R1) receptor subunit downstream from the AAV p40-(pJDT95dlk-aR1) or cytomegalovirus (CMV) promoter (pTRUF3-aR1) in an antisense orientation. Each plasmid drove expression of antisense NMDA-R1 in primary rat neocortical neuronal cultures 4 days after transfection as detected by reverse transcriptase-polymerase chain reaction (RT-PCR). Transfection with pTRUF3-aR1 (2 x 4 mu g) but not with pJDT95dlk-aR1 decreased neuronal [H-3]MK-801 binding in a dose-dependent manner. (C) 1998 Elsevier Science B.V.

引用

页码：325 / 328

页数：4

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