Photo-regulative transfection using a cationic polyazobenzene dendrimer

被引：2

作者：

Atarashi, K ^{[1
]}

Tamagaki, S ^{[1
]}

Nagasaki, T ^{[1
]}

机构：

[1] Osaka City Univ, Fac Engn, Sumiyoshi Ku, Osaka 5588585, Japan

来源：

KOBUNSHI RONBUNSHU | 2000年 / 57卷 / 10期

关键词：

gene delivery; transfection; dendrimer; photo-regulation; azobenzene; zeta potential; vector; polycation;

D O I：

10.1295/koron.57.696

中图分类号：

O63 [高分子化学（高聚物）];

学科分类号：

070305 ; 080501 ; 081704 ;

摘要：

A new cationic L-lysine-modified polyazobenzene dendrimer (Lys-G 2) was examined as a synthetic vector for a mammalian cell. UV irradiation after the incorporation of Lys-G 2-complex with plasmid DNA into a cytoplasm caused a 50% increase in the transfection efficiency as compared with the case without UV irradiation; namely, the trans-to-cis photo-isomerization bad protonated amines serried and decreased the cationic density on the surface of the complex to suppress the cationic repulsion. The decreased charge density was also confirmed by zeta potential measurements. This work demonstrates that the transfection efficiency is controllable by UV irradiation using a cationic dendrimer which possesses a photochromic polyazobenzene structure.

引用

页码：696 / 700

页数：5

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