Biochemical and gene expression analyses of conotoxins in Conus textile venom ducts

被引：30

作者：

Garrett, JE

Buczek, O

Watkins, M

Olivera, BM

Bulaj, G

机构：

[1] Cognetix Inc, Salt Lake City, UT 84108 USA

[2] Univ Utah, Dept Biol, Salt Lake City, UT 84112 USA

[3] Univ Utah, Dept Pathol, Salt Lake City, UT 84112 USA

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2005年 / 328卷 / 01期

关键词：

conotoxins; Conus; cone snails; biosynthesis; conotoxin gene expression; venom duct;

D O I：

10.1016/j.bbrc.2004.12.178

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Each Conus snail species produces 50-200 unique peptide-based conotoxins, derived from a number of different gene superfamilies. Conotoxins are synthesized and secreted in a long venom duct, but biochemical and molecular aspects of their biosynthesis remain poorly understood. Here, we analyzed expression patterns of conotoxin genes belonging to different superfamilies in Conus textile venom ducts. The results demonstrate that specific gene families are expressed in particular regions of the venom duct. Biochemical analysis using liquid chromatography and mass spectrometry revealed an even more localized accumulation of individual conotoxins. This study demonstrates for the first time that specialization of gene expression, processing, and secretion of conotoxins occurs in different regions of the venom duct. (C) 2005 Elsevier Inc. All rights reserved.

引用

收藏

页码：362 / 367

页数：6

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