Comparison of the sensitivities of two Escherichia coli genes to in vivo variation of lrp concentration

被引：8

作者：

Chen, CF ^{[1
]}

Newman, EB ^{[1
]}

机构：

[1] Concordia Univ, Dept Biol, Montreal, PQ H3G 1M8, Canada

来源：

JOURNAL OF BACTERIOLOGY | 1998年 / 180卷 / 03期

关键词：

D O I：

10.1128/JB.180.3.655-659.1998

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Transcription of the Escherichia coli genes serA and gltBDF depends on the leucine-responsive regulatory protein, Lrp, and is very much decreased in an lrp mutant, By the use of an Lrp-deficient host and the lrp gene cloned under a plasmid-borne arabinose pBAD promoter, we varied the amount of Lrp present in the cell and showed that both genes were transcribed in proportion to the amount of Lrp synthesized. The affinity of serA for Lrp was four to five times greater than the affinity of gltD. Overproduction of Lrp was lethal to the cell.

引用

页码：655 / 659

页数：5

共 28 条

[1] ALTERED AMINO-ACID-METABOLISM IN LRP MUTANTS OF ESCHERICHIA-COLI K12 AND THEIR DERIVATIVES [J].

AMBARTSOUMIAN, G ;

DARI, R ;

LIN, RT ;

NEWMAN, EB .

MICROBIOLOGY-UK, 1994, 140 :1737-1744

[2] Use of an in vivo titration method to study a global regulator: Effect of varying Lrp levels on expression of gltBDF in Escherichia coli [J].

Borst, DW ;

Blumenthal, RM ;

Matthews, RG .

JOURNAL OF BACTERIOLOGY, 1996, 178 (23) :6904-6912

[3] THE LEUCINE-RESPONSIVE REGULATORY PROTEIN, A GLOBAL REGULATOR OF METABOLISM IN ESCHERICHIA-COLI [J].

CALVO, JM ;

MATTHEWS, RG .

MICROBIOLOGICAL REVIEWS, 1994, 58 (03) :466-490

[4]

CASTANO IN, 1992, MOL MICROBIOL, V6, P2744

[5] Metabolic regulation of lrp gene expression in Escherichia coli K-12 [J].

Chen, CF ;

Lan, J ;

Korovine, M ;

Shao, ZQ ;

Tao, L ;

Zhang, J ;

Newman, EB .

MICROBIOLOGY-UK, 1997, 143 :2079-2084

[6] A CONSENSUS SEQUENCE FOR BINDING OF LRP TO DNA [J].

CUI, YH ;

WANG, Q ;

STORMO, GD ;

CALVO, JM .

JOURNAL OF BACTERIOLOGY, 1995, 177 (17) :4872-4880

[7] THE LEUCINE-RESPONSIVE REGULATORY PROTEIN - MORE THAN A REGULATOR [J].

DARI, R ;

LIN, RT ;

NEWMAN, EB .

TRENDS IN BIOCHEMICAL SCIENCES, 1993, 18 (07) :260-263

[8] HISTONE-LIKE PROTEINS OF BACTERIA [J].

DRLICA, K ;

ROUVIEREYANIV, J .

MICROBIOLOGICAL REVIEWS, 1987, 51 (03) :301-319

[9] CHARACTERIZATION OF THE REGULON CONTROLLED BY THE LEUCINE-RESPONSIVE REGULATORY PROTEIN IN ESCHERICHIA-COLI [J].

ERNSTING, BR ;

ATKINSON, MR ;

NINFA, AJ ;

MATTHEWS, RG .

JOURNAL OF BACTERIOLOGY, 1992, 174 (04) :1109-1118

[10]

GOTTESMAN S, 1984, ANNU REV GENET, V18, P415

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