IL-1β induced changes in hypothalamic IL-1R1 and IL-1R2 mRNA expression in the rat

被引：12

作者：

Parker, LC ^{[1
]}

Rushforth, DA ^{[1
]}

Rothwell, NJ ^{[1
]}

Luheshi, GN ^{[1
]}

机构：

[1] Univ Manchester, Sch Biol Sci, Manchester M13 9PT, Lancs, England

来源：

MOLECULAR BRAIN RESEARCH | 2000年 / 79卷 / 1-2期

基金：

英国医学研究理事会;

关键词：

rat; IL-1; beta; receptor; hypothalamus; RT-PCR;

D O I：

10.1016/S0169-328X(00)00099-1

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

Interleukin-l receptor (IL-1R1 and IL-1R2) mRNA expression was detected within the rat hypothalamus, a primary site of IL-I action, using RT-PCR. Levels of expression were unchanged by cardiac saline-perfusion. However. intracerebroventricular (i.c.v.) administration of IL-IP caused changes in receptor mRNA expression in non-perfused animals that were profoundly different to those observed in their saline-perfused counterparts. This study demonstrates the importance of perfusing tissue to remove blood cells when determining changes in IL-1 receptor mRNA expression. (C) 2000 Elsevier Science B.V. All rights reserved.

引用

页码：156 / 158

页数：3

共 16 条

[1] Cortical cell death induced by IL-1 is mediated via actions in the hypothalamus of the rat
Allan, SM
Parker, LC
Collins, B
Davies, R
Luheshi, GN
Rothwell, NJ
[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2000, 97 (10) : 5580 - 5585
[2] A RAPID AND VERSATILE METHOD TO SYNTHESIZE INTERNAL STANDARDS FOR COMPETITIVE PCR
CELI, FS
ZENILMAN, ME
SHULDINER, AR
[J]. NUCLEIC ACIDS RESEARCH, 1993, 21 (04) : 1047 - 1047
[3] CENTRAL ENDOTOXIN INDUCES DIFFERENT PATTERNS OF INTERLEUKIN (IL)-1-BETA AND IL-6 MESSENGER-RIBONUCLEIC-ACID EXPRESSION AND IL-6 SECRETION IN THE BRAIN AND PERIPHERY
DESIMONI, MG
DELBO, R
DELUIGI, A
SIMARD, S
FORLONI, G
[J]. ENDOCRINOLOGY, 1995, 136 (03) : 897 - 902
[4] Biologic basis for interleukin-1 in disease
Dinarello, CA
[J]. BLOOD, 1996, 87 (06) : 2095 - 2147
[5] EXPRESSION OF INTERLEUKIN-1-ALPHA, INTERLEUKIN-1-BETA AND INTERLEUKIN-1 RECEPTOR ANTAGONIST MESSENGER-RNA IN MOUSE-BRAIN - REGULATION BY BACTERIAL LIPOPOLYSACCHARIDE (LPS) TREATMENT
GABELLEC, MM
GRIFFAIS, R
FILLION, G
HAOUR, F
[J]. MOLECULAR BRAIN RESEARCH, 1995, 31 (1-2): : 122 - 130
[6] Interleukin-1 receptors type I and type II in the mouse brain: Kinetics of mRNA expressions after peripheral administration of bacterial lipopolysaccharide
Gabellec, MM
Griffais, R
Fillion, G
Haour, F
[J]. JOURNAL OF NEUROIMMUNOLOGY, 1996, 66 (1-2) : 65 - 70
[7] Central nervous system IL-1 beta system and neuropeptide Y mRNAs during IL-1 beta-induced anorexia in rats
Gayle, D
Ilyin, SE
PlataSalaman, CR
[J]. BRAIN RESEARCH BULLETIN, 1997, 44 (03) : 311 - 317
[8] Interleukin-1β system (ligand, receptor type I, receptor accessory protein and receptor antagonist), TNF-α, TGF-β1 and neuropeptide Y mRNAs in specific brain regions during bacterial LPS-induced anorexia
Ilyin, SE
Gayle, D
Flynn, MC
Plata-Salamán, CR
[J]. BRAIN RESEARCH BULLETIN, 1998, 45 (05) : 507 - 515
[9] Endotoxin-induced upregulation of type I interleukin-1 receptor mRNA expression in hepatocytes of mice: Role of cytokines
Ito, A
Takii, T
Soji, T
Onozaki, K
[J]. JOURNAL OF INTERFERON AND CYTOKINE RESEARCH, 1997, 17 (01) : 55 - 61
[10] EXPRESSION OF TYPE-I AND TYPE-II INTERLEUKIN-1 RECEPTORS IN MOUSE-BRAIN
PARNET, P
AMINDARI, S
WU, C
BRUNKEREESE, D
GOUJON, E
WEYHENMEYER, JA
DANTZER, R
KELLEY, KW
[J]. MOLECULAR BRAIN RESEARCH, 1994, 27 (01): : 63 - 70

← 1 2 →