Functional and structural heterogeneity of the DNA binding site of the Escherichia coli primary replicative helicase DnaB protein

The structure-function relationship within the DNA binding site of the Escherichia coli replicative helicase DnaB protein was studied using nuclease digestion, quantitative fluorescence titration, centrifugation, and fluorescence energy transfer techniques, Nuclease digestion of the enzyme-single-stranded DNA (ssDNA) complexes reveals large structural heterogeneity within the binding site, The total site is built of two subsites differing in structure and affinity, although both occlude similar to 10 nucleotides. ssDNA affinity for the strong subsite is similar to 3 orders of magnitude higher than that for the weak subsite, Fluorescence energy transfer experiments provide direct proof that the DnaB hexamer binds ssDNA in a single orientation, with respect to the polarity of the sugar-phosphate backbone, This is the first evidence of directional binding to ssDNA of a hexameric helicase in solution, The strong binding subsite is close to the small 12-kDa domains of the DnaB hexamer and occludes the 5'-end of the ssDNA The strict orientation of the helicase on ssDNA indicates that, when the enzyme approaches the replication fork, it faces double-stranded DNA with its weak subsite. The data indicate that the different binding subsites are located sequentially, with the weak binding subsite constituting the entry site for double-stranded DNA of the replication fork.