Rapid physicochemical detachment, separation and concentration of bacteria from beef surfaces

被引：20

作者：

RodriguezSzulc, UM ^{[1
]}

Ventoura, G ^{[1
]}

Mackey, BM ^{[1
]}

Payne, MJ ^{[1
]}

机构：

[1] FOOD RES INST, READING LAB, READING RG6 6BZ, BERKS, ENGLAND

来源：

JOURNAL OF APPLIED BACTERIOLOGY | 1996年 / 80卷 / 06期

关键词：

D O I：

10.1111/j.1365-2672.1996.tb03273.x

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

A simple, rapid, physicochemical treatment for the removal of viable bacteria from the surface of raw and cooked beef is described. The detachment method was linked to a differential centrifugation step which removed large amounts of particulate food matter and concentrated the detached bacteria. The method increased the numbers of bacteria released from beef surfaces and increased the numbers detected by at least one and a half orders of magnitude, when compared to the traditional 'stomaching' technique. This 1-h separation and concentration method produced cleaner suspensions of bacteria and improved the sensitivity of detection by DEFT and direct plate count.

引用

页码：673 / 681

页数：9

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