High-speed mapping of synaptic connectivity using photostimulation in Channel rhodopsin-2 transgenic mice

被引:286
作者
Wang, H.
Peca, J.
Matsuzaki, M.
Matsuzaki, K.
Noguchi, J.
Qiu, L.
Wang, D.
Zhangn, F.
Boyden, E.
Deisserothl, K.
Kasai, H.
Hall, W. C.
Feng, G.
Augustine, G. J. [1 ]
机构
[1] Duke Univ, Med Ctr, Dept Neurobiol, Durham, NC 27710 USA
[2] Univ Coimbra, Ctr Neurosci & Cell Biol, P-3004517 Coimbra, Portugal
[3] Univ Tokyo, Fac Med, Ctr Dis Biol & Integrat Med, Div Biophys, Tokyo 1130033, Japan
[4] Stanford Univ, Dept Bioengn, Palo Alto, CA 94305 USA
关键词
brain networks; cortical circuitry; synaptic transmission;
D O I
10.1073/pnas.0700384104
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
To permit rapid optical control of brain activity, we have engineered multiple lines of transgenic mice that express the light-activated cation channel Channelrhodopsin-2 (ChR2) in subsets of neurons. Illumination of ChR2-positive neurons in brain slices produced photocurrents that generated action potentials within milliseconds and with precisely timed latencies. The number of light-evoked action potentials could be controlled by varying either the amplitude or duration of illumination. Furthermore, the frequency of light-evoked action potentials could be precisely controlled up to 30 Hz. Photostimulation also could evoke synaptic transmission between neurons, and, by scanning with a small laser light spot, we were able to map the spatial distribution of synaptic circuits connecting neurons within living cerebral cortex. We conclude that ChR2 is a genetically based photostimulation technology that permits analysis of neural circuits with high spatial and temporal resolution in transgenic mammals.
引用
收藏
页码:8143 / 8148
页数:6
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