Evodiamine sensitizes U87 glioblastoma cells to TRAIL via the death receptor pathway

被引:60
作者
Khan, Muhammad [1 ]
Bi, Yanying [2 ]
Qazi, Javed Iqbal [3 ]
Fan, Limei [4 ]
Gao, Hongwen [1 ]
机构
[1] Jilin Univ, Dept Pathol, Hosp 2, Changchun 130041, Peoples R China
[2] Changchun Univ Chinese Med, Affiliated Hosp 1, Infect Control Off, Changchun 130021, Peoples R China
[3] Univ Punjab, Dept Zool, Lahore 54590, Pakistan
[4] Jilin Univ, Dept Gynecol & Obstet, Hosp 2, Changchun 130041, Peoples R China
基金
中国国家自然科学基金;
关键词
evodiamine; TRAIL; U87; cells; DR4; caspase-8; caspase-3; MEDIATED APOPTOSIS; DEPENDENT APOPTOSIS; GLIOMA-CELLS; AUTOPHAGY; DEGRADATION; ARREST;
D O I
10.3892/mmr.2014.2705
中图分类号
R73 [肿瘤学];
学科分类号
100214 [肿瘤学];
摘要
The tumor necrosis factor--related apoptosis-inducing ligand (TRAIL) has been shown to selectively induce death in cancer cells without affecting healthy cells. Most glioma cells are resistant to TRAIL-induced apoptosis. Resistance to TRAIL limits its potential use as a drug for therapy of glioma. The present study was conducted to identify bioactive compounds that have the potential to sensitize U87 glioblastoma cells to TRAIL. Evodiamine, a major bioactive compound of the Chinese herb Evodiae fructus, has been reported to sensitize U87 glioblastoma cells to TRAIL. TRAIL and evodiamine, in combination or alone, were used to treat U87 glioblastoma cells. We show that evodiamine treatment inhibited cell growth in a dose-dependent manner; however, TRAIL alone failed to exert any cytotoxic effect. Combining TRAIL with evodiamine significantly increased the apoptotic rate of U87 glioblastoma cells, as compared to evodiamine treatment alone. Further investigation of the mechanism underlying these effects revealed that the evodiamine + TRAIL effect is associated with the increased expression of death receptor (DR)4, DR5, caspase-8 and cleaved caspase-3. The present study demonstrated, for the first time to the best of our knowledge, that evodiamine can sensitize U87 glioblastoma cells to TRAIL via the death receptor pathway. Thus, our results suggest that combined treatment with evodiamine and TRAIL may represent a novel chemotherapeutic strategy for the therapy of glioma.
引用
收藏
页码:257 / 262
页数:6
相关论文
共 32 条
[1]
Capsaicin-induced apoptosis of glioma cells is mediated by TRPV1 vanilloid receptor and requires p38 MAPK activation [J].
Amantini, C. ;
Mosca, M. ;
Nabissi, M. ;
Lucciarini, R. ;
Caprodossi, S. ;
Arcella, A. ;
Giangaspero, F. ;
Santoni, G. .
JOURNAL OF NEUROCHEMISTRY, 2007, 102 (03) :977-990
[2]
Lanatoside C sensitizes glioblastoma cells to tumor necrosis factor-related apoptosis-inducing ligand and induces an alternative cell death pathway [J].
Badr, Christian E. ;
Wurdinger, Thomas ;
Nilsson, Jonas ;
Niers, Johanna M. ;
Whalen, Michael ;
Degterev, Alexei ;
Tannous, Bakhos A. .
NEURO-ONCOLOGY, 2011, 13 (11) :1213-1224
[3]
Migration of human neural stem cells toward an intracranial glioma [J].
Jeon, Jeong Yong ;
An, Jeung Hee ;
Kim, Seung Up ;
Park, Hye Gyeong ;
Lee, Myung Ae .
EXPERIMENTAL AND MOLECULAR MEDICINE, 2008, 40 (01) :84-91
[4]
Anti-proliferative effects of evodiamine on human prostate cancer cell lines DU145 and PC3 [J].
Kan, Shu-Fen ;
Yu, Ching-Han ;
Pu, Hsiao-Fung ;
Hsu, Jong-Ming ;
Chen, Ming-Jen ;
Wang, Paulus S. .
JOURNAL OF CELLULAR BIOCHEMISTRY, 2007, 101 (01) :44-56
[5]
Keane MM, 1999, CANCER RES, V59, P734
[6]
Evodiamine induces apoptosis in pancreatic carcinoma PANC-1 cells via NF κB inhibition [J].
Khan, Muhammad ;
Qazi, Javed Iqbal ;
Rasul, Azhar ;
Zheng, Yongchen ;
Ma, Tonghui .
BANGLADESH JOURNAL OF PHARMACOLOGY, 2013, 8 (01) :8-14
[7]
Pseudolaric Acid B Induces Caspase-Dependent and Caspase-Independent Apoptosis in U87 Glioblastoma Cells (Publication with Expression of Concern. See vol. 2019, 2019) [J].
Khan, Muhammad ;
Zheng, Bin ;
Yi, Fei ;
Rasul, Azhar ;
Gu, Zhuyi ;
Li, Ting ;
Gao, Hongwen ;
Qazi, Javed Iqbal ;
Yang, Hong ;
Ma, Tonghui .
EVIDENCE-BASED COMPLEMENTARY AND ALTERNATIVE MEDICINE, 2012, 2012
[8]
Alantolactone Induces Apoptosis in HepG2 Cells through GSH Depletion, Inhibition of STAT3 Activation, and Mitochondrial Dysfunction [J].
Khan, Muhammad ;
Li, Ting ;
Khan, Muhammad Khalil Ahmad ;
Rasul, Azhar ;
Nawaz, Faisal ;
Sun, Meiyan ;
Zheng, Yongchen ;
Ma, Tonghui .
BIOMED RESEARCH INTERNATIONAL, 2013, 2013
[9]
Alantolactone induces apoptosis in glioblastoma cells via GSH depletion, ROS generation, and mitochondrial dysfunction [J].
Khan, Muhammad ;
Yi, Fei ;
Rasul, Azhar ;
Li, Ting ;
Wang, Nan ;
Gao, Hongwen ;
Gao, Rong ;
Ma, Tonghui .
IUBMB LIFE, 2012, 64 (09) :783-794
[10]
Isoalantolactone Induces Reactive Oxygen Species Mediated Apoptosis in Pancreatic Carcinoma PANC-1 Cells [J].
Khan, Muhammad ;
Ding, Chuan ;
Rasul, Azhar ;
Yi, Fei ;
Li, Ting ;
Gao, Hongwen ;
Gao, Rong ;
Zhong, Lili ;
Zhang, Kun ;
Fang, Xuedong ;
Ma, Tonghui .
INTERNATIONAL JOURNAL OF BIOLOGICAL SCIENCES, 2012, 8 (04) :533-547