Galactosamine-induced apoptosis in the primary mouse hepatocyte cultures

被引:13
作者
Tsutsui, S [1 ]
Hirasawa, K [1 ]
Takeda, M [1 ]
Itagaki, SI [1 ]
Kawamura, S [1 ]
Maeda, K [1 ]
Mikami, T [1 ]
Doi, K [1 ]
机构
[1] UNIV TOKYO,FAC AGR,DEPT VET MICROBIOL,BUNKYO KU,TOKYO 113,JAPAN
关键词
apoptosis; galactosamine; hepatocyte culture; mouse; cytotoxicity;
D O I
10.1016/S0940-2993(97)80044-9
中图分类号
R36 [病理学];
学科分类号
100104 ;
摘要
Galactosamine (GalN)-induced apoptosis was investigated in cultured hepatocytes from mice. The percentage of fragmented DNA measured by the ELISA method increased in a concentration-dependent manner from the very early stage, i.e. 0.5 hrs, after GalN-exposure. In addition, a ladder-like fragmentation pattern by agarose gel electrophoresis appeared first at 3 hr-exposure tb 20 mM GalN, at 6 hr-exposure to 10 mM GalN and at 12 hr-exposure to 5 mM GalN, respectively. On the other hand, cytotoxicity indicated by leakage of lactate dehydrogenase from cultured hepatocytes to culture medium was first detected at 24 hrs after GalN-treatment. Morphologically, formation of blebs and apoptotic bodies was observed from 22 hr-exposure to 20 mM GalN and from 24 hr-exposure to 10 mM GalN, respectively. Thus GalN could induce apoptosis in primary hepatocyte cultures from mice.
引用
收藏
页码:301 / 306
页数:6
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