Exogenous versus endogenous recovery of 25-hydroxyvitamins D2 and D3 in human samples using high-performance liquid chromatography and the DiaSorin LIAISON Total-D Assay

被引:39
作者
Horst, Ronald L. [1 ]
机构
[1] Heartland Assays Inc, Ames, IA 50010 USA
关键词
Assay; 25-Hydroxyvitamin D; High-performance liquid chromatography; DiaSorin LIAISON; VITAMIN-D;
D O I
10.1016/j.jsbmb.2010.03.010
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Demand for circulating 25-hydroxyvitamin D [25(OH)D] measurements has exploded due to its relationship with many serious health problems. The present study was designed to investigate the validity of samples "spiked" with 25-hydroxyvitamin D-2 [25(OH)D-2] or 25-hydroxyvitamin D-3 [25(OH)D-3] to determine their analytical recovery by the DiaSorin LIAISON 25 OH Vitamin D Total Assay (DiaSorin Assay) and high-performance liquid chromatography (HPLC). 25(OH)D was measured in nine volunteers taking large daily doses of vitamin D-2 for 2 weeks. Samples were obtained pre-supplementation and 1 week following vitamin D. Pre-supplementation samples were used for exogenous recovery studies by adding 25(OH)D-2 or 25(OH)D-3. Endogenous 25(OH)D [25(OH)D-2 plus 25(OH)D-3] concentrations reported by the DiaSorin Assay or detected by HPLC were in excellent agreement. However, exogenously added 25(OH)D-2 and 25(OH)D-3 were under-recovered by the DiaSorin Assay. NIST vitamin D standards containing serum from another species (horse) or exogenous 25(OH)D-2 were similarly affected when using the DiaSorin Assay. Exogenous 25(OH)D-2, 25(OH)D-3 or serum from other species added to human samples is inappropriate in determining the analytical recovery of vitamin D compounds when using the DiaSorin Assay. Only endogenous 25(OH)D-2 and/or 25(OH)D-3 contained in human blood samples should be utilized for this purpose. (C) 2010 Elsevier Ltd. All rights reserved.
引用
收藏
页码:180 / 182
页数:3
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