Endothelial cell proliferation activity in benign prostatic hyperplasia and prostate cancer:: An in vitro model for assessment

被引:25
作者
Weingärtner, K
Ben-Sasson, SA
Stewart, R
Richie, JP
Riedmiller, H
Folklan, J
机构
[1] Childrens Hosp, Dept Surg, Boston, MA 02115 USA
[2] Univ Wurzburg, Dept Urol, Wurzburg, Germany
[3] Harvard Univ, Brigham & Womens Hosp, Sch Med, Dept Surg,Div Urol, Boston, MA 02115 USA
关键词
neovascularization; urine; endothelial growth factors; prostatic hypertrophy; prostatic neoplasms;
D O I
10.1016/S0022-5347(01)63949-1
中图分类号
R5 [内科学]; R69 [泌尿科学(泌尿生殖系疾病)];
学科分类号
1002 ; 100201 ;
摘要
Purpose: Urinary excretion of several pro-angiogenic and antiangiogenic substances has been correlated with malignant tumor growth. The aim of this study was to assay angiogenic activity in urine from patients with cancer of the prostate and benign prostatic hyperplasia (BPH). Materials and Methods: Urine specimens from 22 healthy male volunteers (control), 33 patients with BPH and 29 with organ confined prostate cancer were analyzed for angiogenic activity in a bovine capillary endothelial cell proliferation assay. In parallel the concentration of basic fibroblast growth factor and vascular endothelial growth factor was determined by enzyme immunoassay in the corresponding urine specimens. Results: Urine samples from patients with BPH and prostate cancer increased bovine capillary endothelial cell proliferation by 13.1% and 15.1%, respectively, whereas urine from the control group showed a significantly lower angiogenic activity, increasing endothelial cell proliferation by only 0.7% (p = 0.001). Urinary basic fibroblast growth factor and vascular endothelial growth factor were highest in patients with BPH and lowest in the group with prostate cancer (p = 0.0001). Conclusions: Urine from patients with BPH and prostate cancer stimulates endothelial cell proliferative activity. The degree of endothelial cell stimulation does not correlate with the concentration of basic fibroblast growth factor or vascular endothelial growth factor. Whether the observed pro-angiogenic activity is due to an increased production or release of (an) other angiogenic factor(s) and/or loss of (an) angiogenesis inhibitor(s), deserves further investigation.
引用
收藏
页码:465 / 470
页数:6
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