Genetic and pharmacological evidence for a novel, intermediate phase of long-term potentiation suppressed by calcineurin

被引：287

作者：

Winder, DG ^{[1
]}

Mansuy, IM ^{[1
]}

Osman, M ^{[1
]}

Moallem, TM ^{[1
]}

Kandel, ER ^{[1
]}

机构：

[1] Columbia Univ Coll Phys & Surg, New York State Psychiat Inst, Howard Hughes Med Inst, Ctr Neurobiol & Behav, New York, NY 10032 USA

来源：

CELL | 1998年 / 92卷 / 01期

关键词：

D O I：

10.1016/S0092-8674(00)80896-X

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

To investigate the role of phosphatases in synaptic plasticity using genetic approaches, we generated transgenic mice that overexpress a truncated form of calcineurin under the control of the CaMKII alpha promoter. Mice expressing this transgene show increased calcium-dependent phosphatase activity in the hippocampus. Physiological studies of these mice and parallel pharmacological experiments in wild-type mice reveal a novel, intermediate phase of LTP (I-LTP) in the CA1 region of the hippocampus. This intermediate phase differs from E-LTP by requiring multiple trains for induction and in being dependent on PKA. It differs from L-LTP in not requiring new protein synthesis. These data suggest that calcineurin acts as an inhibitory constraint on I-LTP that is relieved by PKA. This inhibitory constraint acts as a gate to regulate the synaptic induction of L-LTP.

引用

页码：25 / 37

页数：13

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