2′,4′-Dihydroxychalcone-induced apoptosis of human gastric cancer MGC-803 cells via down-regulation of survivin mRNA

被引：33

作者：

Lou, Chenghua ^{[1
,2
]}

Yang, Guangming ^{[1
]}

Cai, Hao ^{[1
]}

Zou, Mingchang ^{[1
]}

Xu, Zisheng ^{[1
,2
]}

Li, Yu ^{[1
]}

Zhao, Fengming ^{[1
]}

Li, Weidong ^{[1
]}

Tong, Li ^{[3
]}

Wang, Mingyan ^{[1
]}

Cai, Baochang ^{[1
,2
]}

机构：

[1] Nanjing Univ Chinese Med, Jiangsu Key Lab Chinese Med Proc, Nanjing 210029, Peoples R China

[2] Nanjing Haichang Chinese Herbal Med Pieces Co Ltd, Nanjing 210061, Peoples R China

[3] Qinghai Univ, Coll Med, Xining 810001, Peoples R China

来源：

TOXICOLOGY IN VITRO | 2010年 / 24卷 / 05期

关键词：

Herba oxytropis; 2; 4; '-Dihydroxychalcone; Apoptosis; Survivin; MGC-803; cells; BREAST-CANCER; ACTIVATION; EXPRESSION; CHALCONES; GENE; FLAVONOIDS; INHIBITION; CARCINOMAS;

D O I：

10.1016/j.tiv.2010.05.003

中图分类号：

R99 [毒物学（毒理学）];

学科分类号：

100405 [卫生毒理学];

摘要：

2',4'-Dihydroxychalcone (TFC), a main component in Herba Oxytropis, is grouped under flavonoids, which are well known to have antitumor activities in vitro. In this study, the possible antitumor mechanism of TFC in human gastric cancer MGC-803 cells is examined. Hoechst 33258 staining analysis indicates that TFC causes MGC-803 cell shrinkage and apoptotic body formation, typical characteristics of apoptosis. Flow cytometric analysis demonstrates that TFC causes cell cycle arrest in the G2/M phase. Furthermore. TFC significantly increases caspase-3 activity but decreases survivin mRNA expression. Therefore, TFC can induce the apoptosis of MGC-803 cells via down-regulation of survivin mRNA expression. (C) 2010 Elsevier Ltd. All rights reserved.

引用

页码：1333 / 1337

页数：5

共 31 条

[1]

A novel anti-apoptosis gene, survivin, expressed in cancer and lymphoma [J].