The Arp2/3 complex branches filament barbed ends: functional antagonism with capping proteins

被引:203
作者
Pantaloni, D
Boujemaa, R
Didry, D
Gounon, P
Carlier, MF [1 ]
机构
[1] CNRS, Lab Enzymol & Biochim Struct, F-91198 Gif Sur Yvette, France
[2] Inst Pasteur, F-75724 Paris, France
关键词
D O I
10.1038/35017011
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
The Arp2/3 complex is an essential regulator of actin polymerization in response to signalling and generates a dendritic array of filaments in lamellipodia. Here we show that the activated Arp2/3 complex interacts with the barbed ends of filaments to initiate barbed-end branching. Barbed-end branching by Arp2/3 quantitatively accounts for polymerization kinetics and for the length correlation of the branches of filaments observed by electron microscopy. Filament branching is Visualized at the surface of Listeria in a reconstituted motility assay. The functional antagonism between the Arp2/3 complex and capping proteins is essential in the maintenance of the steady state of actin assembly and actin-based motility.
引用
收藏
页码:385 / 391
页数:7
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