Reconstitution of the biochemical activities of the AttJ repressor and the AttK, AttL, and AttM catabolic enzymes of Agrobacterium tumefaciens

被引：45

作者：

Chai, Yunrong ^{[1
]}

Tsai, Ching Sung ^{[1
]}

Cho, Hongbaek ^{[1
]}

Winans, Stephen C. ^{[1
]}

机构：

[1] Cornell Univ, Dept Microbiol, Ithaca, NY 14853 USA

来源：

JOURNAL OF BACTERIOLOGY | 2007年 / 189卷 / 09期

关键词：

D O I：

10.1128/JB.01274-06

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The attKLM operon encodes a lactonase (AttM) that hydrolyzes acylhomoserine lactone autoinducers, as well as two putative dehydrogenases (AttK and AttL). Here we show that AttK, AttL, and AttM collectively covert gamma-butyrolactone to succinate. Two metabolic intermediates, gamma-hydroxybutyrate and succinic semi-aldehyde, inactivated the AttJ repressor in vitro and induced attKLM transcription in vivo.

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页码：3674 / 3679

页数：6

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