Osmotic regulation of the mitochondrial permeability transition pore investigated by light scattering, fluorescence and electron microscopy techniques

被引：18

作者：

Baev, Artyom Y. ^{[1
,3
]}

Elustondo, Pia A. ^{[1
]}

Negoda, Alexander ^{[1
]}

Pavlov, Evgeny V. ^{[1
,2
]}

机构：

[1] Dalhousie Univ, Dept Physiol & Biophys, Halifax, NS B3H 4R2, Canada

[2] NYU, Coll Dent, Dept Basic Sci, 345 East 24th St, New York, NY 10010 USA

[3] Educ Expt Ctr High Technol, Tashkent, Uzbekistan

来源：

ANALYTICAL BIOCHEMISTRY | 2018年 / 552卷

关键词：

Mitochondrial permeability transition pore; Calcium; Osmotic pressure; ATP-synthase; Swelling; C-subunit; F1FO ATP SYNTHASE; C-SUBUNIT; INORGANIC POLYPHOSPHATE; MODULATION; CHANNEL; DIMERS; RING;

D O I：

10.1016/j.ab.2017.07.006

中图分类号：

Q5 [生物化学];

学科分类号：

070307 [化学生物学];

摘要：

Mitochondrial permeability transition (PT) is a phenomenon of an increase of the inner membrane permeability in response to an excessive matrix calcium accumulation. PTP is caused by the opening of the large weakly selective channel. Molecular composition and regulation of permeability transition pore (PTP) are not well understood. Here we used isolated mitochondria to investigate dependence of PTP activation on the osmotic pressure. We found that in low osmotic strength solution calcium-induced PTP is significantly inhibited. We propose that this effect, is linked to the changes in the curvature of the mitochondrial inner membrane. This interpretation is consistent with the idea about the importance of ATP synthase dimerization in modulation of the PTP activity. (C) 2017 Published by Elsevier Inc.

引用

页码：38 / 44

页数：7

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