A multiresidual method for the simultaneous determination of the main glycoalkaloids and flavonoids in fresh and processed tomato (Solanum lycopersicum L.) by LC-DAD-MS/MS

被引:10
作者
Dall'Asta, Chiara [1 ]
Falavigna, Claudia [1 ]
Galaverna, Gianni [1 ]
Sforza, Stefano [1 ]
Dossena, Arnaldo [1 ]
Marchelli, Rosangela [1 ]
机构
[1] Univ Parma, Dipartimento Chim Organ & Ind, I-43100 Parma, Italy
关键词
Flavonoids; Glycoalkaloids; LC/MS; Processed tomatoes; Tomatoes; MASS-SPECTROMETRY; ALPHA-TOMATINE; ANTIOXIDANT ACTIVITY; ESCULENTUM; FRUIT; PLANT; LYCOTETRAOSE; CHOLESTEROL; AGLYCONES; GENOTYPES;
D O I
10.1002/jssc.200900365
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
A new multiresidual LC-DAD-MS/MS method for the simultaneous detection of the major flavonoids and glycoalkaloids occurring in tomatoes has been developed and applied to the characterization of seven varieties of raw tomatoes grown in Italy and to their processed products. The new method allowed to determine rapidly the concentration of these secondary metabolites in a single analysis, showing that their content can be quite variable among the different varieties considered. On the contrary, a great depletion in glycoalkaloids and flavonoids is observed after processing, probably due to the harsh thermal treatment, which prevents the recognition of the varieties in the processed products.
引用
收藏
页码:3664 / 3671
页数:8
相关论文
共 32 条
[1]  
Bianco G, 2002, ELECTROPHORESIS, V23, P2904, DOI 10.1002/1522-2683(200209)23:17<2904::AID-ELPS2904>3.0.CO
[2]  
2-1
[3]   Changes in antioxidant and metabolite profiles during production of tomato paste [J].
Capanoglu, Esra ;
Beekwilder, Jules ;
Boyacioglu, Dilek ;
Hall, Robert ;
De Vos, Ric .
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 2008, 56 (03) :964-973
[4]   Analysis of tomato glycoalkaloids by liquid chromatography coupled with electrospray ionization tandem mass spectrometry [J].
Cataldi, TRI ;
Lelario, F ;
Bufo, SA .
RAPID COMMUNICATIONS IN MASS SPECTROMETRY, 2005, 19 (21) :3103-3110
[5]   Quantitative analysis of the flavonoid content of commercial tomatoes, onions, lettuce, and celery [J].
Crozier, A ;
Lean, MEJ ;
McDonald, MS ;
Black, C .
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 1997, 45 (03) :590-595
[6]   Enzyme biosensor for tomatine detection in tomatoes [J].
Dzyadevych, SV ;
Arkhypova, VN ;
Soldatkin, AP ;
El'skaya, AV ;
Martelet, C ;
Jaffrezic-Renault, N .
ANALYTICAL LETTERS, 2004, 37 (08) :1611-1624
[7]   Lowering of plasma LDL cholesterol in hamsters by the tomato glycoalkaloid tomatine [J].
Friedman, M ;
Fitch, TE ;
Yokoyama, WE .
FOOD AND CHEMICAL TOXICOLOGY, 2000, 38 (07) :549-553
[8]   Analysis of biologically active compounds in potatoes (Solanum tuberosum), tomatoes (Lycopersicon esculentum), and jimson weed (Datura stramonium) seeds [J].
Friedman, M .
JOURNAL OF CHROMATOGRAPHY A, 2004, 1054 (1-2) :143-155
[9]   Tomato glycoalkaloids: Role in the plant and in the diet [J].
Friedman, M .
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 2002, 50 (21) :5751-5780
[10]   Flavonoid profiling and biosynthetic gene expression in flesh and peel of two tomato genotypes grown under UV-B-depleted conditions during ripening [J].
Giuntini, Deborah ;
Lazzeri, Valerio ;
Calvenzani, Valentina ;
Dall'Asta, Chiara ;
Galaverna, Gianni ;
Tonelli, Chiara ;
Petroni, Katia ;
Ranieri, Annamaria .
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 2008, 56 (14) :5905-5915