The rrs (16S)-rrl (23S) ribosomal intergenic spacer region as a target for the detection of Haemophilus ducreyi by a heminested-PCR assay

被引：15

作者：

Gu, XX

Rossau, R

Jannes, G

Ballard, R

Laga, M

Van Dyck, E

机构：

[1] Inst Trop Med, Dept Microbiol, B-2000 Antwerp, Belgium

[2] S African Inst Med Res, STD HIV Res Unit, ZA-2000 Johannesburg, South Africa

来源：

MICROBIOLOGY-SGM | 1998年 / 144卷

关键词：

rrs-rrl spacer; Haemophilus ducreyi; chancroid;

D O I：

10.1099/00221287-144-4-1013

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The intergenic spacer region between the rrs and rrl ribosomal RNA genes of Haemophilus ducreyi was analysed and the DNA sequence was used for the selection of specific PCR primers. A highly sensitive and specific heminested-PCR assay for the identification of H. ducreyi was developed. The assay showed a sensitivity of 96% on genital ulcer specimens from patients with clinically diagnosed chancroid, compared with a sensitivity of 56% for culture methods. These results indicate that this PCR assay has the potential to become an accurate and easy reference method for the detection of H. ducreyi.

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页码：1013 / 1019

页数：7

相关论文

共 33 条

[1] MONOCLONAL-ANTIBODIES AGAINST HAEMOPHILUS-DUCREYI LIPOOLIGOSACCHARIDE AND THEIR DIAGNOSTIC USEFULNESS [J].

AHMED, HJ ;

BORRELLI, S ;

JONASSON, J ;

ERIKSSON, L ;

HANSON, S ;

HOJER, B ;

SUNKUNTU, M ;

MUSABA, E ;

ROGGEN, EL ;

LAGERGARD, T ;

LINDBERG, AA .

EUROPEAN JOURNAL OF CLINICAL MICROBIOLOGY & INFECTIOUS DISEASES, 1995, 14 (10) :892-898

[2] Gapped BLAST and PSI-BLAST: a new generation of protein database search programs [J].

Altschul, SF ;

Madden, TL ;

Schaffer, AA ;

Zhang, JH ;

Zhang, Z ;

Miller, W ;

Lipman, DJ .

NUCLEIC ACIDS RESEARCH, 1997, 25 (17) :3389-3402

[3]

Barry T, 1991, PCR Methods Appl, V1, P51

[4]

CHOUDHARY BP, 1982, INDIAN J MED RES, V76, P379

[5] DEVELOPMENT OF THE POLYMERASE CHAIN-REACTION FOR DIAGNOSIS OF CHANCROID [J].

CHUI, L ;

ALBRITTON, W ;

PASTER, B ;

MACLEAN, I ;

MARUSYK, R .

JOURNAL OF CLINICAL MICROBIOLOGY, 1993, 31 (03) :659-664

[6] A SIMPLE MEDIUM FOR THE PRIMARY ISOLATION OF HAEMOPHILUS-DUCREYI [J].

DANGOR, Y ;

MILLER, SD ;

KOORNHOF, HJ ;

BALLARD, RC .

EUROPEAN JOURNAL OF CLINICAL MICROBIOLOGY & INFECTIOUS DISEASES, 1992, 11 (10) :930-934

[7] TRANSPORT MEDIA FOR HAEMOPHILUS-DUCREYI [J].

DANGOR, Y ;

RADEBE, F ;

BALLARD, RC .

SEXUALLY TRANSMITTED DISEASES, 1993, 20 (01) :5-9

[8] ACCURACY OF CLINICAL-DIAGNOSIS OF GENITAL ULCER DISEASE [J].

DANGOR, Y ;

BALLARD, RC ;

EXPOSTO, FD ;

FEHLER, G ;

MILLER, SD ;

KOORNHOF, HJ .

SEXUALLY TRANSMITTED DISEASES, 1990, 17 (04) :184-189

[9] PHYLOGENY OF 54 REPRESENTATIVE STRAINS OF SPECIES IN THE FAMILY PASTEURELLACEAE AS DETERMINED BY COMPARISON OF 16S RIBOSOMAL-RNA SEQUENCES [J].

DEWHIRST, FE ;

PASTER, BJ ;

OLSEN, I ;

FRASER, GJ .

JOURNAL OF BACTERIOLOGY, 1992, 174 (06) :2002-2013

[10] THE CLINICAL-DIAGNOSIS OF GENITAL ULCER DISEASE IN MEN IN THE TROPICS [J].

FAST, MV ;

DCOSTA, LJ ;

NSANZE, H ;

PIOT, P ;

CURRAN, J ;

KARASIRA, P ;

MIRZA, N ;

MACLEAN, IW ;

RONALD, AR .

SEXUALLY TRANSMITTED DISEASES, 1984, 11 (02) :72-76

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