Crystal structure of the Escherichia coli dcm very-short-patch DNA repair endonuclease bound to its reaction product-site in a DNA superhelix

被引:20
作者
Bunting, KA
Roe, SM
Headley, A
Brown, T
Savva, R
Pearl, LH
机构
[1] Inst Canc Res, Chester Beatty Labs, Sect Struct Biol, London SW3 6JB, England
[2] UCL, Dept Biochem & Mol Biol, London WC1E 6BT, England
[3] Univ Southampton, Dept Chem, Southampton SO17 1BJ, Hants, England
[4] Univ London Birkbeck Coll, Mol Biol Lab, Dept Crystallog, London WC1E 7HX, England
基金
英国生物技术与生命科学研究理事会;
关键词
D O I
10.1093/nar/gkg273
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Very-short-patch repair (Vsr) enzymes occur in a variety of bacteria, where they initiate nucleotide excision repair of G:T mismatches arising by deamination of 5-methyl-cytosines in specific regulatory sequences. We have now determined the structure of the archetypal dcm-Vsr endonuclease from Escherichia coli bound to the cleaved authentic hemi-deaminated/hemi-methylated dcm sequence 5'-C-OH-3' 5'-p-T-p-A-p-G-p-G-3'/3'-G-p-G-p-T-p(Me5)C-p-C formed by self-assembly of a 12mer oligonucleotide into a continuous nicked DNA superhelix. The structure reveals the presence of a Hoogsteen base pair within the deaminated recognition sequence and the substantial distortions of the DNA that accompany Vsr binding to product sites.
引用
收藏
页码:1633 / 1639
页数:7
相关论文
共 29 条
  • [1] A Hoogsteen base pair embedded in undistorted B-DNA
    Aishima, J
    Gitti, RK
    Noah, JE
    Gan, HH
    Schlick, T
    Wolberger, C
    [J]. NUCLEIC ACIDS RESEARCH, 2002, 30 (23) : 5244 - 5252
  • [2] Crystal structure of a thwarted mismatch glycosylase DNA repair complex
    Barrett, TE
    Schärer, OD
    Savva, R
    Brown, T
    Jiricny, J
    Verdine, GL
    Pearl, LH
    [J]. EMBO JOURNAL, 1999, 18 (23) : 6599 - 6609
  • [3] Crystal structure of a G:T/U mismatch-specific DNA glycosylase:: Mismatch recognition by complementary-strand interactions
    Barrett, TE
    Savva, R
    Panayotou, G
    Barlow, T
    Brown, T
    Jiricny, J
    Pearl, LH
    [J]. CELL, 1998, 92 (01) : 117 - 129
  • [4] BIOLOGY OF DNA RESTRICTION
    BICKLE, TA
    KRUGER, DH
    [J]. MICROBIOLOGICAL REVIEWS, 1993, 57 (02) : 434 - 450
  • [5] Methylation-induced repression - Belts, braces, and chromatin
    Bird, AP
    Wolffe, AP
    [J]. CELL, 1999, 99 (05) : 451 - 454
  • [6] Crystallography & NMR system:: A new software suite for macromolecular structure determination
    Brunger, AT
    Adams, PD
    Clore, GM
    DeLano, WL
    Gros, P
    Grosse-Kunstleve, RW
    Jiang, JS
    Kuszewski, J
    Nilges, M
    Pannu, NS
    Read, RJ
    Rice, LM
    Simonson, T
    Warren, GL
    [J]. ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY, 1998, 54 : 905 - 921
  • [7] Recognition of GT mismatches by Vsr mismatch endonuclease
    Fox, KR
    Allinson, SL
    Sahagun-Krause, H
    Brown, T
    [J]. NUCLEIC ACIDS RESEARCH, 2000, 28 (13) : 2535 - 2540
  • [8] A new class of uracil-DNA glycosylases related to human thymine-DNA glycosylase
    Gallinari, P
    Jiricny, J
    [J]. NATURE, 1996, 383 (6602) : 735 - 738
  • [9] GLASNER W, 1995, J MOL BIOL, V245, P1
  • [10] DNA binding and cleavage selectivity of the Escherichia coli DNA G:T-mismatch endonuclease (vsr protein)
    Gonzalez-Nicieza, R
    Turner, DP
    Connolly, BA
    [J]. JOURNAL OF MOLECULAR BIOLOGY, 2001, 310 (03) : 501 - 508