Apoptotic Effects of Cordycepin Through the Extrinsic Pathway and p38 MAPK Activation in Human Glioblastoma U87MG Cells

被引：18

作者：

Baik, Ji-Sue ^{[1
,2
,3
]}

Mun, Seo-Won ^{[1
]}

Kim, Kyoung-Sook ^{[1
]}

Park, Shin-Ji ^{[1
,2
,3
]}

Yoon, Hyun-Kyoung ^{[1
]}

Kim, Dong-Hyun ^{[1
]}

Park, Min-Kyu ^{[4
]}

Kim, Cheorl-Ho ^{[5
]}

Lee, Young-Choon ^{[1
]}

机构：

[1] Dong A Univ, Coll Hlth Sci, Dept Med Biotechnol, Busan 49315, South Korea

[2] Dongnam Inst Radiol, Dept Clin Res, Busan 46033, South Korea

[3] Med Sci Res Ctr, Busan 46033, South Korea

[4] Dong A Univ, Coll Med, Dept Clin Pharmacol & Therapeut, Busan 49201, South Korea

[5] Sungkyunkwan Univ, Dept Biol Sci, Mol & Cellular Glycobiol Unit, Kyunggi Do 16419, South Korea

来源：

JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY | 2016年 / 26卷 / 02期

关键词：

Cordycepin; apoptosis; extrinsic pathway; U87MG cell; p38; MAPK; MECHANISMS;

D O I：

10.4014/jmb.1507.07090

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 [微生物学]; 090105 [作物生产系统与生态工程];

摘要：

We first demonstrated that cordycepin inhibited cell growth and triggered apoptosis in U87MG cells with wild-type p53, but not in T98G cells with mutant-type p53. Western blot data revealed that the levels of procaspase-8, -3, and Bcl-2 were downregulated in cordycepin-treated U87MG cells, whereas the levels of Fas, FasL, Bak, cleaved caspase-3, -8, and cleaved PARP were upregulated, indicating that cordycepin induces apoptosis by activating the death receptor-mediated pathway in U87MG cells. Cordycepin-induced apoptosis could be suppressed by only SB203580, a p38 MAPK-specific inhibitor. These results suggest that cordycepin triggered apoptosis in U87MG cells through p38 MAPK activation and inhibition of the Akt survival pathway.

引用

页码：309 / 314

页数：6

共 13 条

[1]

Mammalian MAP kinase signalling cascades [J].