RELT induces cellular death in HEK 293 epithelial cells

被引：40

作者：

Cusick, John K. ^{[1
]}

Mustian, Andrea ^{[2
]}

Goldberg, Kate ^{[1
]}

Reyland, Mary E. ^{[1
]}

机构：

[1] Univ Colorado, Denver Sch Dent Med, Dept Craniofacial Biol, Aurora, CO 80045 USA

[2] Harvard Univ, Sch Dent Med, Boston, MA 02115 USA

来源：

CELLULAR IMMUNOLOGY | 2010年 / 261卷 / 01期

关键词：

Receptor expressed in lymphoid tissues; RELT like protein 1; RELT like protein 2; Tumor Necrosis Factor Receptor; Apoptosis; NECROSIS-FACTOR TNF; FACTOR-RECEPTOR SUPERFAMILY; IDENTIFICATION; ACTIVATION; APOPTOSIS; BIOLOGY; DOMAINS;

D O I：

10.1016/j.cellimm.2009.10.013

中图分类号：

Q2 [细胞生物学];

学科分类号：

071013 [干细胞生物学];

摘要：

RELT is a recently identified Tumor Necrosis Factor Receptor that possess two homologues in humans named RELL1 and RELL2. We investigated whether RELT and its homologues Could induce cellular death when transiently transfected into HEK 293 epithelial cells. Transfection of RELT family members into HEK 293 epithelial cells induced cell death characterized by rounding and lifting of cells accompanied by DNA fragmentation, characteristics that are consistent with the activation of an apoptotic pathway. Overexpression of RELT in COS-7 cells resulted in cell rounding and lifting without DNA fragmentation, suggesting that the effects of RELT signaling may vary among different cell types. In summary, we report that overexpression of RELT or its homologues RELL1 and RELL2 in HEK 293 epithelial cells results in cell death with morphological characteristics consistent with the activation of an apoptotic pathway. (C) 2009 Elsevier Inc. All rights reserved.

引用

页码：1 / 8

页数：8

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