Smoking and oxidant stress: Essay of isoprostane in human urine by gas chromatography-mass spectrometry

被引:58
作者
Obata, T [1 ]
Tomaru, K
Nagakura, T
Izumi, Y
Kawamoto, T
机构
[1] Jikei Univ, Sch Med, Inst DNA Med, Dept Mol Cell Biol, Tokyo 1058461, Japan
[2] Jikei Univ, Sch Med, Dept Pediat, Tokyo, Japan
[3] Univ Occupat & Environm Hlth, Dept Environm Hlth, Fukuoka, Japan
来源
JOURNAL OF CHROMATOGRAPHY B | 2000年 / 746卷 / 01期
关键词
isoprostane;
D O I
10.1016/S0378-4347(00)00182-1
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
Isoprostane (8-epi-prostaglandin F2a) is synthesized non-enzymatically from arachidonate and active oxygen. We examined the relationship of smoking and excretion of isoprostane in urine with gas chromatography-mass spectrometry selected ion monitoring assay and the stable isotope dilution method. Urine isoprostane concentrations were significantly higher in smokers (n=81, 605.24+/-59.01 ng/mg creatinine) than in non-smokers (n=39, 424.07+/-70.37 ng/mg creatinine), but concentrations in ex-smokers (n=21, 487.27+/-98.48 ng/mg creatinine) did not differ significantly from those in the other groups. In smokers, age, the duration of smoking, and the number of cigarettes per day were not correlated with urine isoprostane concentrations. However, urine isoprostane concentrations were negatively correlated with time since quitting in ex-smokers and with age in non-smokers. These results indicate that smoking increases isoprostane concentration in urine and suggest that smoking causes lipid peroxidation by oxidant stress. (C) 2000 Elsevier Science B.V. All rights reserved.
引用
收藏
页码:11 / 15
页数:5
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