Assessment of 1H NMR spectroscopy and multivariate analysis as a technique for metabolite fingerprinting of Arabidopsis thaliana

被引：241

作者：

Ward, JL ^{[1
]}

Harris, C ^{[1
]}

Lewis, J ^{[1
]}

Beale, MH ^{[1
]}

机构：

[1] Univ Bristol, Dept Agr Sci, IACR Long Ashton Res Stn, Bristol BS41 9AF, Avon, England

来源：

PHYTOCHEMISTRY | 2003年 / 62卷 / 06期

基金：

英国生物技术与生命科学研究理事会;

关键词：

Arabidopsis thaliana; NMR spectroscopy; metabolomics; multivariate analysis; principal component analysis; metabolite fingerprints;

D O I：

10.1016/S0031-9422(02)00705-7

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 [生物化学与分子生物学]; 081704 [应用化学];

摘要：

An approach to metabolite fingerprinting of crude plant extracts that utilizes (1)H nuclear magnetic resonance (NMR) spectroscopy and multivariate statistics has been tested. Using ecotypes of Arabidopsis thaliana as experimental material, a method has been developed for the rapid analysis of unfractionated polar plant extracts, enabling the creation of reproducible metabolite fingerprints. These fingerprints could be readily stored and compared by a variety of chemometric methods. Comparison by principal component analysis using SIMCA-P allowed the generation of residual NMR spectra of the compounds that contributed significantly to the differences between samples. From these plots, conclusions were drawn with respect to the identity and relative levels of metabolites differing between samples. (C) 2003 Elsevier Science Ltd. All rights reserved.

引用

页码：949 / 957

页数：9

共 16 条

[1]

Batch statistical processing of 1H NMR-derived urinary spectral data [J].

Antti, H ;

Bollard, ME ;

Ebbels, T ;

Keun, H ;

Lindon, JC ;

Nicholson, JK ;

Holmes, E .

JOURNAL OF CHEMOMETRICS, 2002, 16 (8-10) :461-468

[2]

NMR-based metabonomic studies on the biochemical effects of commonly used drug carrier vehicles in the rat [J].

Beckwith-Hall, BM ;

Holmes, E ;

Lindon, JC ;

Gounarides, J ;

Vickers, A ;

Shapiro, M ;

Nicholson, JK .

CHEMICAL RESEARCH IN TOXICOLOGY, 2002, 15 (09) :1136-1141

[3]

Growth stage-based phenotypic analysis of arabidopsis:: A model for high throughput functional genomics in plants [J].

Boyes, DC ;

Zayed, AM ;

Ascenzi, R ;

McCaskill, AJ ;

Hoffman, NE ;

Davis, KR ;

Görlach, J .

PLANT CELL, 2001, 13 (07) :1499-1510

[4]

Comprehensive chemical profiling of gramineous plant root exudates using high-resolution NMR and MS [J].

Fan, TWM ;

Lane, AN ;

Shenker, M ;

Bartley, JP ;

Crowley, D ;

Higashi, RM .

PHYTOCHEMISTRY, 2001, 57 (02) :209-221

[5]

Metabolite profiling for plant functional genomics [J].

Fiehn, O ;

Kopka, J ;

Dörmann, P ;

Altmann, T ;

Trethewey, RN ;

Willmitzer, L .

NATURE BIOTECHNOLOGY, 2000, 18 (11) :1157-1161

[6]

Metabolomics - the link between genotypes and phenotypes [J].

Fiehn, O .

PLANT MOLECULAR BIOLOGY, 2002, 48 (1-2) :155-171

[7]

Fourier transform infrared spectroscopy and chemometrics as a tool for the rapid detection of other vegetable fats mixed in cocoa butter [J].

Goodacre, R ;

Anklam, E .

JOURNAL OF THE AMERICAN OIL CHEMISTS SOCIETY, 2001, 78 (10) :993-1000

[8]

Hall Robert, 2002, Plant Cell, V14, P1437, DOI 10.1105/tpc.140720

[9]

Chemometric models for toxicity classification based on NMR spectra of biofluids [J].

Holmes, E ;

Nicholls, AW ;

Lindon, JC ;

Connor, SC ;

Connelly, JC ;

Haselden, JN ;

Damment, SJP ;

Spraul, M ;

Neidig, P ;

Nicholson, JK .

CHEMICAL RESEARCH IN TOXICOLOGY, 2000, 13 (06) :471-478

[10]

Analysis of the genome sequence of the flowering plant Arabidopsis thaliana [J].

Kaul, S ;

Koo, HL ;

Jenkins, J ;

Rizzo, M ;

Rooney, T ;

Tallon, LJ ;

Feldblyum, T ;

Nierman, W ;

Benito, MI ;

Lin, XY ;

Town, CD ;

Venter, JC ;

Fraser, CM ;

Tabata, S ;

Nakamura, Y ;

Kaneko, T ;

Sato, S ;

Asamizu, E ;

Kato, T ;

Kotani, H ;

Sasamoto, S ;

Ecker, JR ;

Theologis, A ;

Federspiel, NA ;

Palm, CJ ;

Osborne, BI ;

Shinn, P ;

Conway, AB ;

Vysotskaia, VS ;

Dewar, K ;

Conn, L ;

Lenz, CA ;

Kim, CJ ;

Hansen, NF ;

Liu, SX ;

Buehler, E ;

Altafi, H ;

Sakano, H ;

Dunn, P ;

Lam, B ;

Pham, PK ;

Chao, Q ;

Nguyen, M ;

Yu, GX ;

Chen, HM ;

Southwick, A ;

Lee, JM ;

Miranda, M ;

Toriumi, MJ ;

Davis, RW .

NATURE, 2000, 408 (6814) :796-815

← 1 2 →