Temporal and spatial expression of fibroblast growth factor receptor 4 isoforms in murine tissues

被引:16
作者
Cool, SM [1 ]
Sayer, RE [1 ]
van Heumen, WR [1 ]
Pickles, JO [1 ]
Nurcombe, V [1 ]
机构
[1] Univ Queensland, Sch Biomed Sci, Brisbane, Qld 4072, Australia
来源
HISTOCHEMICAL JOURNAL | 2002年 / 34卷 / 6-7期
基金
英国医学研究理事会; 澳大利亚研究理事会;
关键词
D O I
10.1023/A:1023326524562
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
Fibroblast growth factor receptors (FGFRs) undergo highly regulated spatial and temporal changes of expression during development. This study describes the use of quantitative reverse transcriptase-polymerase chain reaction and immunochemistry to assess the changes in expression of FGFR4 as compared to its FGFR4-17a and -17b isoforms in mouse tissues, from early embryogenesis through to adulthood. Compared to FGFR4, the expression of the isoforms is more restricted at all developmental stages tested. The reverse transcriptase-polymerase chain reaction demonstrated that FGFR4 is expressed in more tissue types than either of its isoforms: it was found predominantly in lung, liver, brain, skeletal muscle and kidney, whereas the FGFR4-17a form was detected in lung and skeletal muscle, and the FGFR4-17b form only in lung, liver, skeletal muscle and kidney. Immunohistochemistry confirmed strong FGFR4-17b expression in the postnatal lung. When combined, the results suggest that FGFR4 variants play important roles particularly in lung and skeletal muscle development.
引用
收藏
页码:291 / 297
页数:7
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