Pollination-enhanced expression of a receptor-like protein kinase related gene in tobacco styles

被引：33

作者：

Li, HY ^{[1
]}

Gray, JE ^{[1
]}

机构：

[1] UNIV SHEFFIELD, DEPT MOL BIOL & BIOTECHNOL, SHEFFIELD S10 2UH, S YORKSHIRE, ENGLAND

来源：

PLANT MOLECULAR BIOLOGY | 1997年 / 33卷 / 04期

关键词：

cDNA; pollination; protein kinase; receptor-like related; style; tobacco;

D O I：

10.1023/A:1005787612041

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We have isolated a putative serine/threonine receptor kinase gene with an expression pattern indicating that it may play a role in the stylar response to pollination. Differential display PCR was used to select tobacco mRNAs with increased accumulation following pollination. NTS16, a cDNA identified by this method, is homologous to a ca. 2.4 kb mRNA primarily expressed in pistil tissues. Levels of this mRNA increase during floral development and are further increased by pollination reaching maximal accumulation 12-18 hours after pollination and then declining. mRNA levels can also be increased by the application of ethylene to unpollinated flowers. A polypeptide encoded by the NTS 16 open reading frame has sequence similarity to the catalytic domain of several receptor protein kinases from plants including the S-receptor kinases implicated in the rejection of self-pollen in Brassica species and the Pto gene product of tomato which confers resistance to a bacterial pathogen.

引用

页码：653 / 665

页数：13

共 65 条

[1]

ALI N, 1994, J BIOL CHEM, V269, P31626

[2] PROTEINASE-INHIBITORS IN NICOTIANA-ALATA STIGMAS ARE DERIVED FROM A PRECURSOR PROTEIN WHICH IS PROCESSED INTO 5 HOMOLOGOUS INHIBITORS [J].

ATKINSON, AH ;

HEATH, RL ;

SIMPSON, RJ ;

CLARKE, AE ;

ANDERSON, MA .

PLANT CELL, 1993, 5 (02) :203-213

[3] ARABIDOPSIS ETHYLENE-RESPONSE GENE ETR1 - SIMILARITY OF PRODUCT TO 2-COMPONENT REGULATORS [J].

CHANG, C ;

KWOK, SF ;

BLEECKER, AB ;

MEYEROWITZ, EM .

SCIENCE, 1993, 262 (5133) :539-544

[4] A FLORAL TRANSMITTING TISSUE-SPECIFIC GLYCOPROTEIN ATTRACTS POLLEN TUBES AND STIMULATES THEIR GROWTH [J].

CHEUNG, AY ;

WANG, H ;

WU, HM .

CELL, 1995, 82 (03) :383-393

[5] SINGLE-STEP METHOD OF RNA ISOLATION BY ACID GUANIDINIUM THIOCYANATE PHENOL CHLOROFORM EXTRACTION [J].

CHOMCZYNSKI, P ;

SACCHI, N .

ANALYTICAL BIOCHEMISTRY, 1987, 162 (01) :156-159

[6] CHARACTERIZATION OF THE S-LOCUS GENES, SLG AND SRK, OF THE BRASSICA S-3 HAPLOTYPE - IDENTIFICATION OF A MEMBRANE-LOCALIZED PROTEIN ENCODED BY THE S-LOCUS RECEPTOR KINASE GENE [J].

DELORME, V ;

GIRANTON, JL ;

HATZFELD, Y ;

FRIRY, A ;

HEIZMANN, P ;

ARIZA, MJ ;

DUMAS, C ;

GAUDE, T ;

COCK, JM .

PLANT JOURNAL, 1995, 7 (03) :429-440

[7] Plant signalling comes of age: Identification of self-pollen in Brassica involves a transmembrane receptor kinase [J].

Dickinson, HG .

TRENDS IN PLANT SCIENCE, 1996, 1 (05) :136-138

[8] TOUCHDOWN PCR TO CIRCUMVENT SPURIOUS PRIMING DURING GENE AMPLIFICATION [J].

DON, RH ;

COX, PT ;

WAINWRIGHT, BJ ;

BAKER, K ;

MATTICK, JS .

NUCLEIC ACIDS RESEARCH, 1991, 19 (14) :4008-4008

[9] A SUPERFAMILY OF S-LOCUS-RELATED SEQUENCES IN ARABIDOPSIS - DIVERSE STRUCTURES AND EXPRESSION PATTERNS [J].

DWYER, KG ;

KANDASAMY, MK ;

MAHOSKY, DI ;

ACCIAI, J ;

KUDISH, BI ;

MILLER, JE ;

NASRALLAH, ME ;

NASRALLAH, JB .

PLANT CELL, 1994, 6 (12) :1829-1843

[10] CLONING AND EXPRESSION OF A DISTINCTIVE CLASS OF SELF-INCOMPATIBILITY (S) GENE FROM PAPAVER-RHOEAS L [J].

FOOTE, HCC ;

RIDE, JP ;

FRANKLINTONG, VE ;

WALKER, EA ;

LAWRENCE, MJ ;

FRANKLIN, FCH .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1994, 91 (06) :2265-2269

← 1 2 3 4 5 6 7 →