Bacterial communities of disease vectors sampled across time, space, and species

被引:41
作者
Jones, Ryan T. [1 ]
Knight, Rob [2 ,3 ]
Martin, Andrew P. [1 ]
机构
[1] Univ Colorado, Dept Ecol & Evolutionary Biol, Boulder, CO 80309 USA
[2] Howard Hughes Med Inst, Chevy Chase, MD USA
[3] Univ Colorado, Dept Chem & Biochem, Boulder, CO 80309 USA
基金
美国国家科学基金会;
关键词
rickettsia; bartonella; community phylogenetics; plague; EARLY-PHASE TRANSMISSION; TAILED PRAIRIE DOGS; COLONIZED CAT FLEAS; YERSINIA-PESTIS; MICROBIAL COMMUNITIES; BARTONELLA-HENSELAE; BORNE RICKETTSIOSES; PLAGUE EPIZOOTICS; UNBLOCKED FLEAS; SIPHONAPTERA;
D O I
10.1038/ismej.2009.111
中图分类号
Q14 [生态学(生物生态学)];
学科分类号
071012 ; 0713 ;
摘要
A common strategy of pathogenic bacteria is to form close associations with parasitic insects that feed on animals and to use these insects as vectors for their own transmission. Pathogens interact closely with other coexisting bacteria within the insect, and interactions between co-occurring bacteria may influence the vector competency of the parasite. Interactions between particular lineages can be explored through measures of alpha-diversity. Furthermore, general patterns of bacterial community assembly can be explored through measures of beta-diversity. Here, we use pyrosequencing (n = 115 924 16S rRNA gene sequences) to describe the bacterial communities of 230 prairie dog fleas sampled across space and time. We use these communinty characterizations to assess interactions between dominant community members and to explore general patterns of bacterial community assembly in fleas. An analysis of co-occurrence patterns suggests non-neutral negative interactions between dominant community members (P < 0.001). Furthermore, bacterial communities of fleas shift dramatically across years (phylotype-based: R = 0.829, P < 0.001; phylogenetic-based: R = 0.612-0.753, P < 0.001), but they also significantly differ across space (phylotype-based: R = 0.418, P < 0.001; phylogenetic-based: R = 0.290-0.328, P < 0.001) and between flea species (phylotype-based: R = 0.160, P = 0.011; phylogenetic-based: not significant). Collectively, our results show that flea-associated bacterial communities are not random assemblages; rather, an individual flea's bacterial community is governed by interactions between bacterial lineages and by the flea's place in space and time. The ISME Journal (2010) 4, 223-231; doi: 10.1038/ismej.2009.111; published online 29 October 2009
引用
收藏
页码:223 / 231
页数:9
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