A simple colorimetric assay for muramic acid and lactic acid

被引：199

作者：

Taylor, KACC

机构：

[1] Department of Animal and Poultry Science, University of Guelph, Guelph, Ont.

来源：

APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY | 1996年 / 56卷 / 01期

关键词：

muramic acid; lactic acid; glyceraldehyde; acetaldehyde; formaldehyde; colorimetric assay;

D O I：

10.1007/BF02787869

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The Barker and Summerson method of assaying lactic acid colorimetrically is modified to provide a simple and fast method of measuring lactic acid and other compounds such as muramic acid and glyceraldehyde that will release acetaldehyde on incubation in hot sulfuric acid. The assay can be done with open tubes and no more complicated equipment than a spectrophotometer. A further modification allows a relatively specific determination of formaldehyde.

引用

页码：49 / 58

页数：10

共 20 条

[1] COLOR-REACTIONS OF CARBONYL-COMPOUNDS WITH SUBSTITUTED PHENOLS .3. FLUOROMETRIC-DETERMINATION OF ACETALDEHYDE AND ITS RELATED COMPOUNDS WITH ORTHO-PHENYLPHENOL [J].