Monoclonal antibodies isolated from human B cells neutralize a broad range of H1 subtype influenza A viruses including swine-origin Influenza virus (S-OIV)

被引：43

作者：

Burioni, Roberto ^{[1
]}

Canducci, Filippo ^{[1
]}

Mancini, Nicasio ^{[1
]}

Clementi, Nicola ^{[1
]}

Sassi, Monica ^{[1
]}

De Marco, Donata ^{[1
]}

Diotti, Roberta Antonia ^{[1
]}

Saita, Diego ^{[1
]}

Sampaolo, Michela ^{[1
]}

Sautto, Giuseppe ^{[1
]}

Pianezze, Matteo ^{[1
]}

Clementi, Massimo ^{[1
]}

机构：

[1] Univ Vita Salute San Raffaele, Lab Microbiol & Virol, I-20132 Milan, Italy

来源：

VIROLOGY | 2010年 / 399卷 / 01期

关键词：

Monoclonal antibodies; Immunotherapy; Pandemics; Influenza; Oseltamivir; Antiviral therapy; IN-VITRO; MOLECULAR CHARACTERIZATION; REPERTOIRE CLONING; NEONATAL FERRETS; H5N1; INFECTION; FAB FRAGMENTS; MICE; PROTECTION; IGG; GLYCOPROTEIN;

D O I：

10.1016/j.virol.2009.12.014

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The new H1N1 swine-origin influenza virus (S-OIV) strain is a global health problem. The elucidation of the virus-host relationship is crucial for the control of the new infection. Two human monoclonal antibody Fab fragments (HMab) neutralizing the novel H1N1 influenza Strain at very low concentrations were cloned before the emergence of S-OIV from a patient who had a broad-range H1N1 serum neutralizing activity. The two HMabs neutralized all tested H1N1 strains, including S-OIV and a swine strain with IC50 ranging from 2 to 7 mu g/ml. Data demonstrate that infection with previously circulating H1N1 strains can elicit antibodies neutralizing S-OIV. Finally, the human genes coding for the neutralizing HMabs could be used for generating full human monoclonal IgGs that can be safely administered being potentially useful in the prophylaxis and the treatment of this human infection. (C) 2009 Elsevier Inc. All rights reserved.

引用

页码：144 / 152

页数：9

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