SARS-CoV Pathogenesis Is Regulated by a STAT1 Dependent but a Type I, II and III Interferon Receptor Independent Mechanism

被引:130
作者
Frieman, Matthew B. [1 ]
Chen, Jun [2 ]
Morrison, Thomas E. [3 ]
Whitmore, Alan [3 ]
Funkhouser, William [4 ]
Ward, Jerrold M. [5 ,6 ]
Lamirande, Elaine W. [2 ]
Roberts, Anjeanette [2 ]
Heise, Mark
Subbarao, Kanta [2 ]
Baric, Ralph S. [1 ,3 ]
机构
[1] Univ N Carolina, Dept Epidemiol, Chapel Hill, NC USA
[2] NIAID, Infect Dis Lab, NIH, Bethesda, MD 20892 USA
[3] Univ N Carolina, Dept Microbiol & Immunol, Chapel Hill, NC USA
[4] Univ N Carolina, Dept Anat Pathol & Surg Pathol, Chapel Hill, NC USA
[5] NIAID, Comparat Med Branch, NIH, Bethesda, MD 20892 USA
[6] NIAID, Immunopathol Lab, NIH, Bethesda, MD 20892 USA
关键词
ACUTE RESPIRATORY SYNDROME; CORONAVIRUS CAUSES DISEASE; INNATE IMMUNITY; HOST-DEFENSE; INFECTION; MICE; IFN; REPLICATION; RESPONSES; PROTEIN;
D O I
10.1371/journal.ppat.1000849
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Severe acute respiratory syndrome coronavirus (SARS-CoV) infection often caused severe end stage lung disease and organizing phase diffuse alveolar damage, especially in the elderly. The virus-host interactions that governed development of these acute end stage lung diseases and death are unknown. To address this question, we evaluated the role of innate immune signaling in protection from human (Urbani) and a recombinant mouse adapted SARS-CoV, designated rMA15. In contrast to most models of viral pathogenesis, infection of type I, type II or type III interferon knockout mice (129 background) with either Urbani or MA15 viruses resulted in clinical disease outcomes, including transient weight loss, denuding bronchiolitis and alveolar inflammation and recovery, identical to that seen in infection of wildtype mice. This suggests that type I, II and III interferon signaling play minor roles in regulating SARS pathogenesis in mouse models. In contrast, infection of STAT1(-/-) mice resulted in severe disease, high virus titer, extensive pulmonary lesions and 100% mortality by day 9 and 30 post-infection with rMA15 or Urbani viruses, respectively. Non-lethal in BALB/c mice, Urbani SARS CoV infection in STAT1(-/-) mice caused disseminated infection involving the liver, spleen and other tissues after day 9. These findings demonstrated that SARS-CoV pathogenesis is regulated by a STAT1 dependent but type I, II and III interferon receptor independent, mechanism. In contrast to a well documented role in innate immunity, we propose that STAT1 also protects mice via its role as an antagonist of unrestrained cell proliferation.
引用
收藏
页码:1 / 14
页数:14
相关论文
共 53 条
  • [1] STAT-1 signaling in human lung fibroblasts is induced by vanadium pentoxide through an IFN-β autocrine loop
    Antao-Menezes, Aurita
    Turpin, Elizabeth A.
    Bost, Phillip C.
    Ryman-Rasmussen, Jessica P.
    Bonner, James C.
    [J]. JOURNAL OF IMMUNOLOGY, 2008, 180 (06) : 4200 - 4207
  • [2] Genomic analysis reveals age-dependent innate immune responses to severe acute respiratory syndrome coronavirus
    Baas, Tracey
    Roberts, Anjeanette
    Teal, Thomas H.
    Vogel, Leatrice
    Chen, Jun
    Tumpey, Terrence M.
    Katze, Michael G.
    Subbarao, Kanta
    [J]. JOURNAL OF VIROLOGY, 2008, 82 (19) : 9465 - 9476
  • [3] The Ebola virus VP35 protein functions as a type IIFN antagonist
    Basler, CF
    Wang, XY
    Mühlberger, E
    Volchkov, V
    Paragas, J
    Klenk, HD
    Garcia-Sastre, A
    Palese, P
    [J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2000, 97 (22) : 12289 - 12294
  • [4] The role of the Type I interferon response in the resistance of mice to filovirus infection
    Bray, M
    [J]. JOURNAL OF GENERAL VIROLOGY, 2001, 82 : 1365 - 1373
  • [5] The role of STATs in transcriptional control and their impact on cellular function
    Bromberg, J
    Darnell, JE
    [J]. ONCOGENE, 2000, 19 (21) : 2468 - 2473
  • [6] Interferon-mediated immunopathological events are associated with atypical innate and adaptive immune responses in patients with severe acute respiratory syndrome
    Cameron, Mark J.
    Ran, Longsi
    Xu, Luoling
    Danesh, Ali
    Bermejo-Martin, Jesus F.
    Cameron, Cheryl M.
    Muller, Matthew P.
    Gold, Wayne L.
    Richardson, Susan E.
    Poutanen, Susan M.
    Willey, Barbara M.
    DeVries, Mark E.
    Fang, Yuan
    Seneviratne, Charit
    Bosinger, Steven E.
    Persad, Desmond
    Wilkinson, Peter
    Greller, Larry D.
    Somogyi, Roland
    Humar, Atul
    Keshavjee, Shaf
    Louie, Marie
    Loeb, Mark B.
    Brunton, James
    McGeer, Allison J.
    Kelvin, David J.
    [J]. JOURNAL OF VIROLOGY, 2007, 81 (16) : 8692 - 8706
  • [7] SARS: Epidemiology
    Chan-Yeung, M
    Xu, RH
    [J]. RESPIROLOGY, 2003, 8 : S9 - S14
  • [8] Epidemiological determinants of spread of causal agent of severe acute respiratory syndrome in Hong Kong
    Donnelly, CA
    Ghani, AC
    Leung, GM
    Hedley, AJ
    Fraser, C
    Riley, S
    Abu-Raddad, LJ
    Ho, LM
    Thach, TQ
    Chau, P
    Chan, KP
    Lam, TH
    Tse, LY
    Tsang, T
    Liu, SH
    Kong, JHB
    Lau, EMC
    Ferguson, NM
    Anderson, RM
    [J]. LANCET, 2003, 361 (9371) : 1761 - 1766
  • [9] Identification of a novel coronavirus in patients with severe acute respiratory syndrome
    Drosten, C
    Günther, S
    Preiser, W
    van der Werf, S
    Brodt, HR
    Becker, S
    Rabenau, H
    Panning, M
    Kolesnikova, L
    Fouchier, RAM
    Berger, A
    Burguière, AM
    Cinatl, J
    Eickmann, M
    Escriou, N
    Grywna, K
    Kramme, S
    Manuguerra, JC
    Müller, S
    Rickerts, V
    Stürmer, M
    Vieth, S
    Klenk, HD
    Osterhaus, ADME
    Schmitz, H
    Doerr, HW
    [J]. NEW ENGLAND JOURNAL OF MEDICINE, 2003, 348 (20) : 1967 - 1976
  • [10] Type IIFN modulates innate and specific antiviral immunity
    Durbin, JE
    Fernandez-Sesma, A
    Lee, CK
    Rao, TD
    Frey, AB
    Moran, TM
    Vukmanovic, S
    García-Sastre, A
    Levy, DE
    [J]. JOURNAL OF IMMUNOLOGY, 2000, 164 (08) : 4220 - 4228