Heat shock-induced matrix metal loproteinase (MMP)-1 and MMP-3 are mediated through ERK and JNK activation and via an autocrine interleukin-6 loop

被引:125
作者
Park, CH
Lee, MJ
Ahn, J
Kim, S
Kim, HH
Kim, KH
Eun, HC
Chung, JH [2 ]
机构
[1] Seoul Natl Univ Hosp, Clin Res Inst, Lab Cutaneous Aging Res, Seoul 110744, South Korea
[2] Seoul Natl Univ, Coll Med, Dept Dermatol, Seoul, South Korea
关键词
ERK; heat shock; interleukin-6; JNK; MMP-1; MMP-3;
D O I
10.1111/j.0022-202X.2004.23487.x
中图分类号
R75 [皮肤病学与性病学];
学科分类号
100206 ;
摘要
Although many studies have been performed to elucidate the molecular consequences of ultraviolet irradiation, little is known about the effect of infrared radiation on skin aging. In addition to photons, heat is likely to be generated as a consequence of infrared irradiation, and heat shock is widely considered to be an environmental stress. Here we investigated the effect of heat shock on the expressions of matrix metalloproteinase (MMP)-1, MMP-2, and MMP-3 in cultured human skin fibroblasts. Heat shock induced the expression of MMP-1 and MMP-3, but not MMP-2, at the mRNA and protein levels in a temperature-de pendent manner, and caused the rapid activation of three distinct mitogen-activated protein kinases (MAPK), extracelluar signal-regulated kinase (ERK), c-Jun N-terminal kinase (JNK), and p38 MAPK. The heat shock-induced MMP-1 and MMP-3 expression was suppressed by the inhibition of ERK and JNK but not by p38 MAPK inhibition. Furthermore, heat shock increased the synthesis and release of interleukin-6 (IL-6) into culture media. The specific inhibition of IL-6 using a monoclonal antibody against IL-6 greatly reduced the expression of MMP-1 and MMP-3 induced by heat shock. Taken together, our results suggest that ERK and JNK play an important role in the induction of MMP-1 and MMP-3 by heat shock and that the heat shock-induced expression of MMP-1 and MMP-3 is mediated via an IL-6-dependent autocrine mechanism.
引用
收藏
页码:1012 / 1019
页数:8
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