Generation of Naive Induced Pluripotent Stem Cells from Rhesus Monkey Fibroblasts

被引：145

作者：

Fang, Riguo ^{[1
,2
]}

Liu, Kang ^{[1
,2
]}

Zhao, Yang ^{[1
,2
]}

Li, Haibo ^{[2
]}

Zhu, Dicong ^{[2
,3
]}

Du, Yuanyuan ^{[1
,2
]}

Xiang, Chengang ^{[1
,2
]}

Li, Xiang ^{[1
,2
]}

Liu, Haisong ^{[2
,3
]}

Miao, Zhenchuan ^{[4
]}

Zhang, Xing ^{[1
,2
]}

Shi, Yan ^{[1
,3
]}

Yang, Weifeng ^{[4
]}

Xu, Jun ^{[1
,2
]}

Deng, Hongkui ^{[1
,2
,3
]}

机构：

[1] Peking Univ, Peking Tsinghua Ctr Life Sci, Coll Life Sci, MOE Key Lab Cell Proliferat & Differentiat, Beijing 100871, Peoples R China

[2] Peking Univ, Hlth Sci Ctr, Stem Cell Res Ctr, Dept Cell Biol,Sch Basic Med Sci, Beijing 100191, Peoples R China

[3] Peking Univ, Shenzhen Grad Sch, Key Lab Chem Genom, Shenzhen Stem Cell Engn Lab, Shenzhen 518055, Peoples R China

[4] Beijing Vitalstar Biotechnol, Beijing 100012, Peoples R China

来源：

CELL STEM CELL | 2014年 / 15卷 / 04期

基金：

中国国家自然科学基金;

关键词：

MAINTAINS PLURIPOTENCY; TRANSCRIPTION FACTOR; GROUND-STATE; DERIVATION; PATHWAYS; INDUCTION; NANOG; LINES;

D O I：

10.1016/j.stem.2014.09.004

中图分类号：

Q813 [细胞工程];

学科分类号：

100113 [医学细胞生物学];

摘要：

Conventional embryonic stem cells (ESCs) or induced pluripotent stem cells (iPSCs) derived from primates resemble mouse epiblast stem cells, raising an intriguing question regarding whether the naive pluripotent state resembling mouse embryonic stem cells (mESCs) exists in primates and how to capture it in vitro. Here we identified several specific signaling modulators that are sufficient to generate rhesus monkey fibroblast-derived iPSCs with the features of naive pluripotency in terms of growth properties, gene expression profiles, self-renewal signaling, X-reactivation, and the potential to generate cross-species chimeric embryos. Interestingly, together with recent reports of naive human pluripotent stem cells, our findings suggest several conserved signaling pathways shared with rodents and specific to primates, providing significant in-sights for acquiring naive pluripotency from other species. In addition, the derivation of rhesus monkey naive iPSCs also provides a valuable cell source for use in preclinical research and disease modeling.

引用

页码：488 / 496

页数：9

共 37 条

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FORMATION OF GERM-LINE CHIMERAS FROM EMBRYO-DERIVED TERATOCARCINOMA CELL-LINES [J].