Transcriptional hierarchy in Xenopus embryogenesis: HNF4 a maternal factor involved in the developmental activation of the gene encoding the tissue specific transcription factor HNF1 alpha (LFB1)

被引：38

作者：

Holewa, B ^{[1
]}

vonStrandmann, EP ^{[1
]}

Zapp, D ^{[1
]}

Lorenz, P ^{[1
]}

Ryffel, GU ^{[1
]}

机构：

[1] UNIV ESSEN GESAMTHSCH KLINIKUM, INST ZELLBIOL TUMORFORSCH, D-45122 ESSEN, GERMANY

来源：

MECHANISMS OF DEVELOPMENT | 1996年 / 54卷 / 01期

关键词：

transcriptional cascade; maternal gradient; gene injection; promoter; nuclear receptor;

D O I：

10.1016/0925-4773(95)00460-2

中图分类号：

Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

The tissue specific transcription factor HNF1 alpha (LFB1) expressed in liver, kidney, stomach and gut gets transcriptionally activated in Xenopus shortly after zygotic transcription starts. By microinjection into fertilized Xenopus eggs, a HNF1 alpha promoter fragment is activated in the middle part of developing larvae, reflecting the activation pattern of the endogenous HNF1 alpha gene. Mutational analysis of the HNF1 alpha promoter shows that HNF1 and HNF4 binding sites are essential for proper embryonic regulation. Since by injecting HNF4 mRNA into fertilized eggs the endogenous HNF1 alpha gene is activated ectopically and HNF4 is present as a maternal protein within an animal to vegetal gradient in the embryo, we assume that HNF4 initiates a transcriptional hierarchy involved in determination of different cell fates.

引用

页码：45 / 57

页数：13

共 47 条

[1] DEVELOPMENTAL REGULATION AND TISSUE DISTRIBUTION OF THE LIVER TRANSCRIPTION FACTOR LFB1 (HNF1) IN XENOPUS-LAEVIS [J].

BARTKOWSKI, S ;

ZAPP, D ;

WEBER, H ;

EBERLE, G ;

ZOIDL, C ;

SENKEL, S ;

KLEINHITPASS, L ;

RYFFEL, GU .

MOLECULAR AND CELLULAR BIOLOGY, 1993, 13 (01) :421-431

[2] HNF-1 SHARES 3 SEQUENCE MOTIFS WITH THE POU DOMAIN PROTEINS AND IS IDENTICAL TO LF-B1 AND APF [J].

BAUMHUETER, S ;

MENDEL, DB ;

CONLEY, PB ;

KUO, CJ ;

TURK, C ;

GRAVES, MK ;

EDWARDS, CA ;

COURTOIS, G ;

CRABTREE, GR .

GENES & DEVELOPMENT, 1990, 4 (03) :372-379

[3]

BEARER EL, 1994, EUR J CELL BIOL, V63, P255

[4]

BLUMENFELD M, 1991, DEVELOPMENT, V113, P589

[5]

BOUWMEESTER T, 1994, ONCOGENE, V9, P1029

[6] HEPATOCYTE DEDIFFERENTIATION AND EXTINCTION IS ACCOMPANIED BY A BLOCK IN THE SYNTHESIS OF MESSENGER-RNA CODING FOR THE TRANSCRIPTION FACTOR HNF1/LFB1 [J].

CEREGHINI, S ;

YANIV, M ;

CORTESE, R .

EMBO JOURNAL, 1990, 9 (07) :2257-2263

[7] DISRUPTION OF THE HNF-4 GENE, EXPRESSED IN VISCERAL ENDODERM, LEADS TO CELL-DEATH IN EMBRYONIC ECTODERM AND IMPAIRED GASTRULATION OF MOUSE EMBRYOS [J].

CHEN, WS ;

MANOVA, K ;

WEINSTEIN, DC ;

DUNCAN, SA ;

PLUMP, AS ;

PREZIOSO, VR ;

BACHVAROVA, RF ;

DARNELL, JE .

GENES & DEVELOPMENT, 1994, 8 (20) :2466-2477

[8] MOLECULAR NATURE OF SPEMANNS ORGANIZER - THE ROLE OF THE XENOPUS HOMEOBOX GENE GOOSECOID [J].

CHO, KWY ;

BLUMBERG, B ;

STEINBEISSER, H ;

DEROBERTIS, EM .

CELL, 1991, 67 (06) :1111-1120

[9]

DAWID IB, 1994, J BIOL CHEM, V269, P6259

[10] CLONING AND DEVELOPMENTAL EXPRESSION OF LFB3/HNF1-BETA TRANSCRIPTION FACTOR IN XENOPUS-LAEVIS [J].

DEMARTIS, A ;

MAFFEI, M ;

VIGNALI, R ;

BARSACCHI, G ;

DESIMONE, V .

MECHANISMS OF DEVELOPMENT, 1994, 47 (01) :19-28

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