Optimization of Supercritical Fluid Extraction of Oil from the Fruit of Gardenia jasminoides and Its Antidepressant Activity

被引：58

作者：

Tao, Weiwei ^{[1
,2
,3
]}

Zhang, Hailou ^{[1
,2
]}

Xue, Wenda ^{[1
,2
]}

Ren, Li ^{[1
,2
]}

Xia, Baomei ^{[1
,2
]}

Zhou, Xin ^{[1
,2
]}

Wu, Haoxin ^{[1
,2
]}

Duan, Jinao ^{[3
]}

Chen, Gang ^{[1
,2
]}

机构：

[1] Nanjing Univ Chinese Med, Sch Basic Biomed Sci, Ctr Translat Syst Biol & Neurosci, Nanjing 210023, Jiangsu, Peoples R China

[2] Nanjing Univ Chinese Med, Sch Basic Biomed Sci, Lab Integrat Biomed Brain Dis, Nanjing 210023, Jiangsu, Peoples R China

[3] Nanjing Univ Chinese Med, Natl & Local Collaborat Engn Ctr Chinese Med Reso, Nanjing 210023, Jiangsu, Peoples R China

来源：

MOLECULES | 2014年 / 19卷 / 12期

基金：

中国国家自然科学基金;

关键词：

supercritical fluid extraction; response surface methodology; oil; Gardenia jasminoides; antidepressant effect; GENIPOSIDE; FRUCTUS; STRESS; MODELS; ELLIS;

D O I：

10.3390/molecules191219350

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

070307 [化学生物学]; 071010 [生物化学与分子生物学];

摘要：

A response surface methodology was applied to optimize the variables affecting the supercritical fluid carbon dioxide extraction of oil from the fruit of Gardenia jasminoides using the Box-Behnken design. The optimum extraction parameters were an extraction temperature of 49.94 degrees C, an extraction pressure of 29.89 MPa and an extraction time of 93.82 min. Through a GC/MS analysis, we revealed 16 major components of the oil extract, which showed potent antidepressant effects in both of two behavior despair models in mice: tail suspension test and forced swimming test. Our results suggest that the oil extract of Gardenia jasminoides prepared using the supercritical fluid carbon dioxide extraction may contain effective constituents to be used for depression therapy.

引用

页码：19350 / 19360

页数：11

共 16 条

[1]

Melanogenesis Inhibitory Activity of Monoterpene Glycosides from Gardeniae Fructus [J].